Department of Otorhinolaryngology, Head and Neck Surgery.
Institute of Medical Microbiology, University Hospital Essen, University Duisburg-Essen, Essen.
Otol Neurotol. 2018 Dec;39(10):e985-e991. doi: 10.1097/MAO.0000000000002021.
Biofilm formation on cochlear implant (CI) surfaces differs between bacterial species and can be reduced by the application of S53P4 bioactive glass.
The formation of bacterial biofilms on medical devices, such as cochlear implants, can lead to chronic infections resulting in the need for implant removal. In this study, various surfaces of three CI implant kits from different manufacturers were examined for bacterial biofilm formation and reduction of a pre-existing biofilm by the application of bioactive glass.
Biofilm formations of 4 bacterial species causing implant-related infections were tested on 17 different surfaces: Pseudomonas aeruginosa (ATCC9027), Staphylococcus aureus (ATCC6538), Staphylococcus epidermidis (ATCC12228), and Streptococcus pyogenes (ATCC19615). For P. aeruginosa and S. aureus biofilm reduction after application of S53P4 bioactive glass was evaluated.
All tested microbial species formed biofilms on the examined CI surfaces in a strain-dependent manner. For S. aureus, a significantly higher biofilm formation on metal components compared with silicone was found whereas the other strains did not show a material specific biofilm formation. Application of S53P4 bioactive glass resulted in a significant reduction of P. aeruginosa and S. aureus mature biofilm.
The four bacteria species displayed biofilm formation on the CI surfaces in a species- and material-specific manner. The results show that bioactive glass can reduce biofilm formation on CI materials in vitro. Future studies are necessary to confirm the results in vivo.
不同细菌物种在耳蜗植入物（CI）表面形成生物膜的情况不同，并且可以通过应用 S53P4 生物活性玻璃来减少。
在医疗器械（如耳蜗植入物）上形成细菌生物膜会导致慢性感染，从而需要取出植入物。在这项研究中，研究人员检查了来自不同制造商的三种 CI 植入套件的各种表面，以研究细菌生物膜的形成以及通过应用生物活性玻璃减少已存在的生物膜。
在 17 种不同的表面上测试了导致植入物相关感染的 4 种细菌物种的生物膜形成：铜绿假单胞菌（ATCC9027）、金黄色葡萄球菌（ATCC6538）、表皮葡萄球菌（ATCC12228）和化脓性链球菌（ATCC19615）。还评估了在应用 S53P4 生物活性玻璃后对铜绿假单胞菌和金黄色葡萄球菌生物膜减少的效果。
所有测试的微生物物种都以菌株依赖性的方式在检查的 CI 表面上形成生物膜。对于金黄色葡萄球菌，与硅酮相比，金属组件上的生物膜形成明显更高，而其他菌株则没有表现出特定材料的生物膜形成。应用 S53P4 生物活性玻璃可显著减少铜绿假单胞菌和金黄色葡萄球菌成熟生物膜的形成。
四种细菌物种以物种和材料特异性的方式在 CI 表面上形成生物膜。结果表明，生物活性玻璃可以在体外减少 CI 材料上的生物膜形成。需要进一步的研究来确认体内结果。
