Henan Orthopedic Institute, Henan Luoyang Orthopedic-Traumatological Hospital (Henan Orthopedic Hospital), Luoyang 471000, China.
Biomed Res Int. 2020 Sep 28;2020:7473942. doi: 10.1155/2020/7473942. eCollection 2020.
External socking and washing with the Chinese herbal Sanhuang Jiedu decoction (SHJD) can effectively control local limb infections with bone and implant exposure. However, the antibiofilm activities of this decoction have not yet been investigated. Therefore, the aim of this study was to examine the effects and characteristics of SHJD on the mature biofilms of multidrug-resistant on a titanium surface.
Biofilm-forming methicillin-resistant 35984 and 43330, and non-biofilm-forming 12228 were selected as the experimental strains. The mature biofilms were prepared on titanium surfaces. The five experimental groups were based on dilution concentrations (DC) of SHJD: the control group (biofilm incubated with 0.85% NaCl solution), the SHJD (DC:1/8) group (initial SHJD solution was diluted 1/8), the SHJD (DC:1/4) group, the SHJD (DC:1/2) group, and the SHJD (DC:1/1) group (initial SHJD solution). The effects of SHJD on the mature biofilms were observed with the bacterial spread plate method, crystal violet (CV) staining, scanning electron microscopy, and confocal laser scanning microscopy.
After culture in tryptic soy broth for 72 h, 43300 and 35984 produced mature biofilms and 12228 did not. The optical density value of 12228 was 0.11 ± 0.02, significantly lower than that of 35984 (0.42 ± 0.05) or 43300 (0.41 ± 0.03) ( < 0.05). The mature biofilms of 43300 and 35984 clearly disintegrated when incubated for 12-24 h with SHJD (DC:1/1) or SHJD (DC:1/2), showing only scattered bacterial adhesion. In the SHJD (DC:1/4) group, although many residual bacterial colonies still clustered together, presenting a biofilm structure, it was very looser than that in the SHJD (DC:1/8) group in which the biofilm was similar to that in the control group. For 12228, only colony adhesion was observed, and the number of colonies decreased as the concentration of SHJD or the culture period increased. The quantitative results for the bacterial spread plate and CV staining showed significant differences between the SHJD groups ( < 0.05).
SHJD has antibiofilm activity against multidrug-resistant strains. It weakens or disrupts already-formed mature biofilms on titanium surfaces in a concentration- and incubation time-dependent manner.
外用包裹和中药三黄解毒汤（SHJD）洗涤可有效控制骨和植入物外露部位的局部肢体感染。然而，这种方剂的抗生物膜活性尚未得到研究。因此，本研究旨在研究 SHJD 对钛表面上耐多药 成熟生物膜的作用和特性。
选择耐甲氧西林 35984 和 43330 以及非生物膜形成 12228 作为实验菌株。在钛表面上制备成熟的生物膜。根据 SHJD 的稀释浓度（DC）将五个实验组分为：对照组（用 0.85%NaCl 溶液孵育的生物膜），SHJD（DC：1/8）组（初始 SHJD 溶液稀释 1/8），SHJD（DC：1/4）组，SHJD（DC：1/2）组和 SHJD（DC：1/1）组（初始 SHJD 溶液）。通过细菌平板扩散法、结晶紫（CV）染色、扫描电子显微镜和共聚焦激光扫描显微镜观察 SHJD 对成熟生物膜的影响。
在胰蛋白酶大豆肉汤中培养 72 小时后，43300 和 35984 产生成熟的生物膜，而 12228 则没有。12228 的光密度值为 0.11 ± 0.02，明显低于 35984（0.42 ± 0.05）或 43300（0.41 ± 0.03）（<0.05）。用 SHJD（DC：1/1）或 SHJD（DC：1/2）孵育 12-24 小时后，43300 和 35984 的成熟生物膜明显崩解，仅显示散在的细菌黏附。在 SHJD（DC：1/4）组中，虽然仍有许多残留的细菌菌落聚集在一起，呈现生物膜结构，但与 SHJD（DC：1/8）组相比，其生物膜结构非常松散，而 SHJD（DC：1/8）组的生物膜结构与对照组相似。对于 12228，仅观察到菌落黏附，并且随着 SHJD 的浓度或培养时间的增加，菌落数量减少。细菌平板扩散和 CV 染色的定量结果显示 SHJD 组之间存在显著差异（<0.05）。
SHJD 对耐多药 菌株具有抗生物膜活性。它以浓度和孵育时间依赖的方式削弱或破坏钛表面上已经形成的成熟生物膜。
