Scaria A, Edenberg H J
Mutat Res. 1987 May;183(3):265-71. doi: 10.1016/0167-8817(87)90009-5.
We are examining the effects of preirradiation of host (monkey) cells upon the replication of UV-damaged SV40. Control cells and cells preirradiated with low fluences (5 or 10 J/m2) of UV were infected with undamaged SV40, and the immediate effects of a subsequent irradiation were determined. UV inhibited total SV 40 DNA synthesis (incorporation of thymidine into viral DNA) in both preirradiated and control cells, but the extent of inhibition was less in the preirradiated cells. A test fluence of 60 J/m2 to SV40 replicating in preirradiated cells reduced synthesis only as much as a test fluence of 25 J/m2 in control cells. The fraction of recently replicated SV40 molecules that re-entered the replication pool and subsequently completed one round of replication in the first 2 h after UV was also decreased less in the preirradiated cells. Thus preirradiation of the host cell mitigates the immediate inhibitory effects of a subsequent UV exposure upon SV40 replication.
我们正在研究宿主（猴）细胞的预照射对紫外线损伤的SV40复制的影响。用未损伤的SV40感染对照细胞和用低剂量（5或10 J/m2）紫外线预照射的细胞，并确定随后照射的即时效应。紫外线抑制了预照射细胞和对照细胞中总的SV40 DNA合成（胸苷掺入病毒DNA），但预照射细胞中的抑制程度较小。在预照射细胞中复制的SV40，用60 J/m2的测试剂量照射，其合成减少程度仅相当于对照细胞中25 J/m2测试剂量照射的减少程度。在预照射细胞中，紫外线照射后最初2小时内重新进入复制池并随后完成一轮复制的最近复制的SV40分子比例的降低也较少。因此，宿主细胞的预照射减轻了随后紫外线暴露对SV40复制的即时抑制作用。
