苯巴比妥和6-氨基烟酰胺对不同大鼠脑区与能量代谢相关的脑酶活性的影响。

Phenobarbital and 6-aminonicotinamide effect on cerebral enzymatic activities related to energy metabolism in different rat brain areas.

作者信息

Marzatico F, Dagani F, Curti D, Benzi G

出版信息

Neurochem Res. 1987 Jan;12(1):33-9. doi: 10.1007/BF00971361.

Abstract

The effect of phenobarbital (100 mg/kg i.p.) and 6-aminonicotinamide (6AN) (35 mg/kg i.p.) on enzyme activities related to energy transduction was investigated on the homogenate "in toto", non-synaptic mitochondrial fraction and synaptosomal fraction isolated from different rat brain areas (cerebral cortex, hippocampus, hypothalamus, striatum, and medulla oblongata). 6AN treatment decreased: phosphofructokinase in all the areas tested; lactate dehydrogenase on the homogenate "in toto" in striatum and hypothalamus, and on the synaptosomal fraction in cerebral cortex and corpus striatum; succinate dehydrogenase on non-synaptic mitochondrial fraction in hippocampus and striatum. Finally, aspartate aminotransferase was increased on non-synaptic mitochondrial fraction in striatum and medulla oblongata. Phenobarbital treatment induced an increase of total NADH cytochrome c reductase on mitochondrial fraction in hippocampus and hypothalamus, and a decrease of cytochrome oxidase activity on non-synaptic mitochondrial fraction in hypothalamus and medulla oblongata.

摘要

研究了苯巴比妥（腹腔注射100mg/kg）和6-氨基烟酰胺（6AN，腹腔注射35mg/kg）对从不同大鼠脑区（大脑皮层、海马体、下丘脑、纹状体和延髓）分离得到的全脑组织匀浆、非突触线粒体组分和突触体组分中与能量转导相关的酶活性的影响。6-氨基烟酰胺处理降低了：所有测试脑区中的磷酸果糖激酶；纹状体和下丘脑全脑组织匀浆中以及大脑皮层和纹状体突触体组分中的乳酸脱氢酶；海马体和纹状体非突触线粒体组分中的琥珀酸脱氢酶。最后，纹状体和延髓非突触线粒体组分中的天冬氨酸转氨酶增加。苯巴比妥处理导致海马体和下丘脑线粒体组分中总NADH细胞色素c还原酶增加，以及下丘脑和延髓非突触线粒体组分中细胞色素氧化酶活性降低。