School of Biological Science, Luoyang Normal University, Luoyang 471934, China; Cold water fish breeding Engineering Technology Research Center of Henan province, Luoyang 471934, China.
School of Biological Science, Luoyang Normal University, Luoyang 471934, China.
Toxicol Appl Pharmacol. 2019 Jan 1;362:35-42. doi: 10.1016/j.taap.2018.10.014. Epub 2018 Oct 16.
Triclosan (TCS) is a broad-spectrum antibacterial agent and has been widely used in a diversity of personal care products. However, recent studies suggest that TCS has some adverse effects, and some evidences suggest in vitro neurotoxicity and developmental neurotoxicity of embryos. There are currently few studies concerning the mechanisms of TCS induced late developmental neurotoxic effect. Therefore, we investigated effects of juvenile zebrafish (Danio rerio) exposure to TCS. Male juvenile zebrafish were exposed to 68.0 μg/L TCS for 42 days. After the exposure experiment, eight Agilent Zebrafish V3 Gene Expression Microarrays were used to identify gene expression changes on brains from the control and TCS treated zebrafish. Microarray analysis yielded 364 differentially expressed genes (FDR adjusted P-value <.05; fold change >2) predominately represented by visual perception, immune system process, multicellular organismal development, antigen processing and presentation, macrophage differentiation functional categories. Pathway analysis showed that TCS mainly influenced Phototransduction and Cytokine-cytokine receptor interaction. In addition, visual perception functional genes involved in Phototransduction were upregulated, while immune system process functional genes involved in Cytokine-cytokine receptor interaction were downregulated. Quantitative real-time PCR (qRT-PCR) analysis confirmed the microarray data. These data suggest that TCS could affect visual centers, immune system, and development in the brain of juvenile fish to cause central neurotoxicity. Our study reveals new molecular targets for TCS and provides further insights into the molecular mechanisms of TCS toxicity during late development.
三氯生（TCS）是一种广谱抗菌剂，已广泛应用于多种个人护理产品。然而，最近的研究表明 TCS 具有一些不良影响，并且一些证据表明其具有体外神经毒性和胚胎发育神经毒性。目前关于 TCS 诱导的晚期发育神经毒性作用的机制研究较少。因此，我们研究了 TCS 对幼年斑马鱼的影响。雄性幼年斑马鱼暴露于 68.0μg/L TCS 中 42 天。暴露实验结束后，使用 8 个 Agilent Zebrafish V3 Gene Expression Microarrays 来识别对照组和 TCS 处理组斑马鱼大脑中的基因表达变化。微阵列分析产生了 364 个差异表达基因（FDR 调整的 P 值<.05；倍数变化>2），主要代表视觉感知、免疫系统过程、多细胞生物发育、抗原加工和呈递、巨噬细胞分化功能类别。通路分析表明 TCS 主要影响光转导和细胞因子-细胞因子受体相互作用。此外，参与光转导的视觉感知功能基因上调，而参与细胞因子-细胞因子受体相互作用的免疫系统过程功能基因下调。定量实时 PCR（qRT-PCR）分析证实了微阵列数据。这些数据表明 TCS 可能会影响幼年鱼类大脑中的视觉中心、免疫系统和发育，从而导致中枢神经毒性。我们的研究揭示了 TCS 的新分子靶标，并为 TCS 毒性在晚期发育过程中的分子机制提供了进一步的见解。
