Easson Garrett, Laughlin Megan, Jensen Hanna, Haney Kevin, Girardot Marc, Jensen Morten
1 Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR, USA.
2 Ozark Regional Vein Center, Rogers, AR, USA.
Phlebology. 2019 Jun;34(5):347-354. doi: 10.1177/0268355518804360. Epub 2018 Oct 18.
The purpose of this study is to test venous valve performance and identify differences between native tissue and replacement devices developed with traditional tissue treatment methods using a new in vitro model with synchronized hemodynamic parameters and high-speed valve image acquisition.
An in vitro model mimicking the venous circulation to test valve performance was developed using hydrostatic pressure driven flow. Fresh and glutaraldehyde-treated vein segments were placed in the setup and opening/closing of the valves was captured by a high-speed camera. Hemodynamic data were obtained using synchronized hardware and virtual instrumentation.
Geometric orifice area and opening/closing time of the valves was evaluated at the same hemodynamic conditions. A reduction in geometric orifice area of 27.2 ± 14.8% (p < 0.05) was observed following glutaraldehyde fixation. No significant difference in opening/closing time following chemical fixation was observed.
The developed in vitro model was shown to be an effective method for measuring the performance of venous valves. The observed decrease in geometric orifice area following glutaraldehyde treatment indicates a decrease in flow through the valve, demonstrating the consequences of traditional tissue treatment methods.
本研究旨在使用一种具有同步血流动力学参数和高速瓣膜图像采集功能的新型体外模型，测试静脉瓣膜性能，并确定天然组织与采用传统组织处理方法开发的置换装置之间的差异。
利用静水压力驱动血流，开发了一种模拟静脉循环以测试瓣膜性能的体外模型。将新鲜的和经戊二醛处理的静脉段置于该装置中，通过高速摄像机捕捉瓣膜的开闭情况。使用同步硬件和虚拟仪器获取血流动力学数据。
在相同的血流动力学条件下评估瓣膜的几何开口面积和开闭时间。戊二醛固定后，观察到几何开口面积减少了27.2±14.8%（p<0.05）。化学固定后，开闭时间无显著差异。
所开发的体外模型被证明是测量静脉瓣膜性能的有效方法。戊二醛处理后观察到的几何开口面积减小表明通过瓣膜的血流减少，这证明了传统组织处理方法的后果。
