Long Y Z, Liu S Y, Li W, Dong Y M
Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
Department of Dental Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2018 Oct 18;50(5):887-891.
To investigate the physical and chemical properties of pulp capping materials based on bioactive glass (BG).
Novel BG pulp capping materials were composed of powder and fluid. The powder was BG (82.36% SiO, 15.36% CaO, and 2.28% PO) synthesized by using the sol-gel method combined with template technology. Two kinds of fluid were provided: (1) phosphate buffer (PB) solution and (2) phosphate buffer solution with 1% sodium alginate (SA) addition. After mixing the powder and fluid, BG-PB and BG-PB-SA were prepared. Setting time and compressive strength of the BG pulp capping materials were tested by setting time loading system and mechanical testing machine. Statistical analysis was performed using the independent sample t-test, with the significance set at 0.05. pH meters was used to test the pH of the BG pulp capping materials and mineral trioxide aggregate (MTA). The sealing ability of the BG pulp capping materials and MTA was tested by methylene blue dye leakage model. Statistical analysis was performed using one-way ANOVA analysis and LSD multiple comparison, with the significance set at 0.05.
(1) Setting time: the initial and final setting time of BG-PB were (7.2±0.3) min and (12.7±0.9) min, respectively. And the initial and final setting time of BG-PB-SA was (7.5±0.3) min and (13.6±1.6) min. There was no significant difference between BG-PB and BG-PB-SA groups (P>0.05). (2) Compressive strength: the compressive strength of BG-PB was (16.5±1.8) MPa at 1 day and (14.1±3.7) MPa at the end of 28 days. However, the compressive strength of BG-PB-SA was (26.6±6.3) MPa on day 1 and (21.6±5.6) MPa on day 28, which was significantly higher than that of BG-PB (P<0.05). (3) pH: the pH of BG pulp capping materials' bulk immersed in simulated body fluid (SBF) went up to 8.06, and the highest pH of MTA was 8.47. Significant difference was observed between the BG pulp capping materials and MTA (P<0.05). (4) Sealing ability: the optical density (D) in positive control group was significantly higher than ln BG-PB, BG-PB-SA and MTA groups (P<0.05). And BG-PB and BG-PB-SA showed the similar favorable sealing ability with MTA, and no significant difference was observed among the three groups (P>0.05).
The novel BG pulp capping materials showed good physical properties, especially BG's setting time was short; BG pulp capping materials are promising.
研究基于生物活性玻璃（BG）的牙髓盖髓材料的物理和化学性质。
新型BG牙髓盖髓材料由粉末和液体组成。粉末为采用溶胶-凝胶法结合模板技术合成的BG（82.36%二氧化硅、15.36%氧化钙和2.28%五氧化二磷）。提供两种液体：（1）磷酸盐缓冲（PB）溶液和（2）添加1%海藻酸钠（SA）的磷酸盐缓冲溶液。将粉末与液体混合后，制备出BG-PB和BG-PB-SA。通过凝固时间加载系统和机械试验机测试BG牙髓盖髓材料的凝固时间和抗压强度。采用独立样本t检验进行统计分析，显著性水平设定为0.05。使用pH计测试BG牙髓盖髓材料和三氧化矿物凝聚体（MTA）的pH值。通过亚甲蓝染料渗漏模型测试BG牙髓盖髓材料和MTA的封闭能力。采用单因素方差分析和LSD多重比较进行统计分析，显著性水平设定为0.05。
（1）凝固时间：BG-PB的初凝时间和终凝时间分别为（7.2±0.3）分钟和（12.7±0.9）分钟。BG-PB-SA的初凝时间和终凝时间分别为（7.5±0.3）分钟和（13.6±1.6）分钟。BG-PB组和BG-PB-SA组之间无显著差异（P>0.05）。（2）抗压强度：BG-PB在1天时的抗压强度为（16.5±1.8）兆帕，在28天时为（14.1±3.7）兆帕。然而，BG-PB-SA在第1天的抗压强度为（26.6±6.3）兆帕，在第28天为（21.6±5.6）兆帕，显著高于BG-PB（P<0.05）。（3）pH值：浸入模拟体液（SBF）中的BG牙髓盖髓材料本体的pH值升至8.06，MTA的最高pH值为8.47。BG牙髓盖髓材料与MTA之间存在显著差异（P<0.05）。（4）封闭能力：阳性对照组的光密度（D）显著高于BG-PB、BG-PB-SA和MTA组（P<0.05）。BG-PB和BG-PB-SA与MTA表现出相似的良好封闭能力，三组之间未观察到显著差异（P>0.05）。
新型BG牙髓盖髓材料显示出良好的物理性能，尤其是BG的凝固时间短；BG牙髓盖髓材料具有应用前景。
