The effect of phosphodiesterase-5 inhibitor, tadalafil, on potassium chloride-induced contractions of isolated human ureteral tissue.

INTRODUCTION

Drugs causing ureteral relaxation are used for medical expulsive therapy (MET) for stones. We investigated the ability of tadalafil to cause relaxation of potassium chloride (KCl)-induced contractions of isolated human ureteral tissue.

MATERIALS AND METHODS

Eight grossly normal proximal ureteral tissues were collected from the radical and donor nephrectomy specimen. The standard organ bath protocol was followed. Ureteral contractions were induced with 80 mM KCl before and after exposure to tadalafil.

RESULTS

The median amplitude and frequency of KCl-induced contractions and the median area under the contractility curve (AUCC) after exposure to 20 μM tadalafil showed significant reductions compared to that of before exposure to tadalafil (7.87 cm, 3.79/min, and 2.98 cm, respectively, versus 9.37 cm, 4.48/min, and 4.50 cm, respectively; = 0,026, 0.008, and 0.008, respectively). After exposure to 40 μM tadalafil, the median amplitude and frequency of KCl-induced contractions and AUCC (4.50 cm, 2.56/min, and 0.92 cm, respectively) showed significant reductions compared to that of before exposure to tadalafil (7.62 cm, 3.88/min, and 3.32 cm, respectively; = 0.008, 0.016, and 0.008, respectively). However, reductions in the parameters after exposure to 20 μM and 40 μM tadalafil were similar ( = 0.065, 0.195, and 0.130, respectively, for median amplitude, frequency, and AUCC).

CONCLUSION

Tadalafil reduces KCl-induced contractions of isolated human ureteral tissue . No incremental relaxations in contractions occurred by increasing the dose of tadalafil from 20 μM to 40 μM.