在单纯疱疹病毒1型基因组的S组分中，对培养生长非必需的基因聚类。

Clustering of genes dispensable for growth in culture in the S component of the HSV-1 genome.

作者信息

Longnecker R, Roizman B

出版信息

Science. 1987 May 1;236(4801):573-6. doi: 10.1126/science.3033823.

Abstract

The herpes simplex virus 1 genome consists of one long and one short stretch of unique sequences flanked by inverted repeat sequences. The nucleotide sequence and RNA map predict 12 open reading frames designated as US1 through US12 within the short stretch of unique sequences. This paper reports the construction of virus mutants from which US2, US3, or US4 had been deleted that are capable of growth in cell culture. One of the three deleted genes, US4, specifies the viral envelope glycoprotein G. Mutants with deletions in US1, US8, US9, US10, US11, and US12 have been previously reported. The nine genes deleted from this region form two clusters, US1 through US4 and US8 through US12, and encode at least two and possibly more structural proteins. The presence of so many genes dispensable for growth in cell culture suggests several hypotheses regarding their function and evolution.

摘要

单纯疱疹病毒1型基因组由一段长的和一段短的独特序列组成，两侧为反向重复序列。核苷酸序列和RNA图谱预测，在短的独特序列区域内有12个开放阅读框，命名为US1至US12。本文报道了构建缺失US2、US3或US4的病毒突变体，这些突变体能够在细胞培养中生长。三个缺失基因之一的US4编码病毒包膜糖蛋白G。先前已报道了在US1、US8、US9、US10、US11和US12中存在缺失的突变体。从该区域缺失的九个基因形成两个簇，即US1至US4和US8至US12，并编码至少两种可能更多的结构蛋白。在细胞培养中生长可有如此多的基因缺失，这提示了关于它们的功能和进化的几种假说。