Umene K, Yoshida M
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Arch Virol. 1989;106(3-4):281-99. doi: 10.1007/BF01313958.
The stability of regions containing tandemly reiterated sequences in the S component of the herpes simplex virus type 1 (HSV-1) genome was determined, by comparing restriction fragments of the regions among sets of HSV-1 isolates derived from a single source. The 6 reiterations examined were grouped into three. Reiteration VII (within protein coding regions of genes US10 and US11) and reiteration IV (within introns of genes US1 and US12) were stable between the isolates (group 1). Regions containing one of four other reiterations were detected as a set of ladder-like fragments. Reiteration II (between "a" sequence and IE 175 gene) and reiteration VI (within an intergenic region on the 3' side of the 3' co-terminal family of genes US10, US11, and US12) (group 3) were more unstable than reiteration I (within "a" sequence) and reiteration III (between "a" sequence and IE175 gene) (group 2). The mode of fluctuation of the reiterations observed within a set of HSV-1 strains isolated from an individual was similar to that observed between HSV-1 single-plaque clones separated in cultured cells. These reiterations, except for group 3, can serve as sensitive and convenient markers for differentiating HSV-1 strains.
通过比较来自单一来源的单纯疱疹病毒1型（HSV-1）分离株中这些区域的限制性片段，确定了HSV-1基因组S组分中含有串联重复序列区域的稳定性。所检测的6个重复序列被分为三组。重复序列VII（在基因US10和US11的蛋白质编码区域内）和重复序列IV（在基因US1和US12的内含子内）在分离株之间是稳定的（第1组）。含有其他四个重复序列之一的区域被检测为一组梯状片段。重复序列II（在“a”序列和IE 175基因之间）和重复序列VI（在基因US10、US11和US12的3'共末端家族3'侧的基因间区域内）（第3组）比重复序列I（在“a”序列内）和重复序列III（在“a”序列和IE175基因之间）（第2组）更不稳定。从个体分离的一组HSV-1毒株中观察到的重复序列波动模式与在培养细胞中分离的HSV-1单斑克隆之间观察到的模式相似。除第3组外，这些重复序列可作为区分HSV-1毒株的敏感且方便的标记。
