Yang Li, Zeng Mulan, Du Yue, Wang Li, Peng Bingxian
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang, China.
Luminescence. 2018 Nov;33(7):1268-1274. doi: 10.1002/bio.3545. Epub 2018 Sep 14.
A ratiometric fluorescence sensor for Cu detection was developed by employing carbon dots (CDs) as the reference fluorophore, and bovine serum albumin (BSA)-Au nanoclusters (AuNCs) both as specific recognition element and as response signal. The CDs were prepared by heating an acetic acid solution of chitosan at 180°C. The proposed nanoprobe was prepared by simply mixing the CDs and BSA-AuNCs. Here, the CDs were associated with BSA-AuNCs to form CDs/BSA-AuNCs nanoprobes based on electrostatic interaction and hydrogen bonds. In the hybrid fluorescent CDs/BSA-AuNCs nanoprobe, the emission of BSA-AuNCs at 640 nm was quenched by Cu, whereas the emission of CDs at 424 nm was maintained. The fluorescence changes of I/I had good linearity with Cu concentration in the range 1.5-600 nM, and the detection limit of the nanoprobe was 0.5 nM for Cu detection, almost 4 × 10 times lower than the maximum allowable amount of Cu in drinking water. The proposed method for detecting Cu also had high selectivity and feasibility.
通过使用碳点（CDs）作为参比荧光团，牛血清白蛋白（BSA）-金纳米簇（AuNCs）既作为特异性识别元件又作为响应信号，开发了一种用于检测铜的比率荧光传感器。CDs是通过在180°C加热壳聚糖的乙酸溶液制备的。所提出的纳米探针是通过简单地将CDs和BSA-AuNCs混合制备的。在此，CDs与BSA-AuNCs基于静电相互作用和氢键形成CDs/BSA-AuNCs纳米探针。在混合荧光CDs/BSA-AuNCs纳米探针中，640nm处BSA-AuNCs的发射被铜淬灭，而424nm处CDs的发射保持不变。I/I的荧光变化与1.5 - 600 nM范围内的铜浓度具有良好的线性关系，该纳米探针检测铜的检测限为0.5 nM，几乎比饮用水中铜的最大允许量低4×10倍。所提出的检测铜的方法还具有高选择性和可行性。
