Guangdong Food and Drug Vocational College, Longdongbei Road 321, Tianhe District, Guangzhou 510520, China.
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.
Molecules. 2018 Oct 18;23(10):2677. doi: 10.3390/molecules23102677.
Tea () cultivars with green leaves are the most widely used for making tea. Recently, tea mutants with white or yellow young shoots have attracted increasing interest as raw materials for making "high-quality" tea products. Albino teas are generallycharacterized as having metabolites of relatively high amino acid content and lower catechin content. However, little is known about aroma compounds in albino tea leaves. Herein, we compared original normal leaves (green) and light-sensitive albino leaves (yellow) of cv. Yinghong No. 9. GC-MS was employed to analyze endogenous tea aroma compounds and related precursors. Quantitative real time PCR was used to measure expression levels of genes involved in biosyntheses of tea aromas.The total contents of most endogenous free tea aromas, including aroma fatty acid derivatives, aroma terpenes, and aroma phenylpropanoids/benzenoids, and their glycosidically bound aroma compounds, were lower in yellow leaves than in green leaves. The content of the key precursor geranyl diphosphate (GDP) and expression levels of key synthetic genes involved in the formation of linalool, a major aroma compound in cv. Yinghong No. 9, were investigated. Linalool content was lower in albino-induced yellow leaves, which was due to the lower GDP content compared with normal green leaves.
具有绿叶的茶树品种是最常用于制作茶叶的。最近,具有白色或黄色新芽的茶树突变体作为制作“高质量”茶产品的原料引起了越来越多的关注。白化茶通常的特征是具有相对较高的氨基酸含量和较低的儿茶素含量的代谢物。然而,关于白化茶叶中的香气化合物知之甚少。在此,我们比较了 cv. 映红 9 号的原始正常叶片(绿色)和光敏感白化叶片(黄色)。GC-MS 用于分析内源性茶香气化合物及相关前体。采用定量实时 PCR 测量参与茶香气生物合成的基因的表达水平。大多数内源性游离茶香气的总量,包括香气脂肪酸衍生物、香气萜类化合物和香气苯丙烷类/苯类,以及它们的糖苷结合香气化合物,在黄色叶片中的含量低于绿色叶片。研究了关键前体香叶基二磷酸 (GDP) 的含量和参与 cv. 映红 9 号中主要香气化合物芳樟醇形成的关键合成基因的表达水平。由于与正常绿色叶片相比 GDP 含量较低,白化诱导的黄色叶片中的芳樟醇含量较低。