Yang Danni, Yang Yi, Li Yongxin, Yin Shuo, Chen Yaling, Wang Jiamin, Xiao Jiangyu, Sun Chengjun
Sichuan University, West China School of Public Health, Chengdu 610041, China.
J AOAC Int. 2019 May 1;102(3):942-951. doi: 10.5740/jaoacint.18-0160. Epub 2018 Oct 19.
Consistent toxicological evidence indicate that phthalates can cause adverse effects on human health. The concern over phthalate pollution and exposure has been emphasized in recent years. Therefore, the sensitive, reliable, and rapid detection of phthalates in water is of great importance. In this study, dispersive liquid-liquid microextraction based on solidification of floating organic droplet (DLLME-SFO) combined with HPLC-UV detection was established and applied in the preconcentration and detection of 15 phthalates in drinking and river water samples. A mixture of acetonitrile (dispersant) and 1-dodecanol (extractant) was injected into water samples, which had been added with sodium chloride. The cloudy solution was formed by hand-shaking. After centrifugation, the sample solution was cooled in a refrigerator, and the solidified organic droplet was collected. It melted at room temperature and was injected into the HPLC system for analysis. The quantification was based on the working curves. Under optimum conditions, this method showed good linearity in the range of 0.1-100 or 0.5-100 μg/L with correlation coefficients greater than 0.999. The method had the LODs ranging from 0.013 to 0.16 μg/L with the enrichment factors of 102-218. The recoveries of the method ranged from 86.8 to 119% with RSDs less than 12.6%. The interday and intraday RSDs were 6.35-13.5% and 3.00-13.7%, respectively. The established method has been successfully applied to the analysis of phthalates in drinking and river waters. The established method is rapid, sensitive, cost-effective, and environmentally friendly. It can be applied to the analysis of 15 phthalates in drinking and river water samples. A method of DLLME-SFO combined with HPLC-UV detection has been established for the analysis of 15 phthalates in drinking and river water samples. The established method was rapid, sensitive, accurate, cost-effective, and environmentally friendly. The established method was successfully applied to the analysis of 15 phthalates in bottled, tap, and river water samples.
一致的毒理学证据表明，邻苯二甲酸盐可对人体健康产生不利影响。近年来，对邻苯二甲酸盐污染和暴露的关注不断增加。因此，灵敏、可靠且快速地检测水中的邻苯二甲酸盐至关重要。在本研究中，基于漂浮有机液滴固化的分散液液微萃取（DLLME-SFO）结合高效液相色谱-紫外检测被建立，并应用于饮用水和河水样品中15种邻苯二甲酸盐的预浓缩和检测。将乙腈（分散剂）和1-十二醇（萃取剂）的混合物注入已添加氯化钠的水样中。通过手摇形成浑浊溶液。离心后，将样品溶液在冰箱中冷却，收集固化的有机液滴。它在室温下融化并注入高效液相色谱系统进行分析。定量基于工作曲线。在最佳条件下，该方法在0.1-100或0.5-100μg/L范围内具有良好的线性，相关系数大于0.999。该方法的检测限为0.013至0.16μg/L，富集因子为102-218。该方法的回收率为86.8至119%，相对标准偏差小于12.6%。日间和日内相对标准偏差分别为6.35-13.5%和3.00-13.7%。所建立的方法已成功应用于饮用水和河水中邻苯二甲酸盐的分析。所建立的方法快速、灵敏、经济高效且环境友好。它可应用于饮用水和河水样品中15种邻苯二甲酸盐的分析。已建立了一种DLLME-SFO结合高效液相色谱-紫外检测的方法，用于分析饮用水和河水样品中的15种邻苯二甲酸盐。所建立的方法快速、灵敏、准确、经济高效且环境友好。所建立的方法已成功应用于瓶装水、自来水和河水样品中15种邻苯二甲酸盐的分析。
