Shirahata T, Shimizu K, Noda S, Suzuki N
Z Parasitenkd. 1977 Aug 25;53(1):31-40. doi: 10.1007/BF00383112.
In vitro assessments were carried out to study some biological aspects of immune lymphocytes producing a new lymphokine, called by the authors Toxoplasma growth inhibitory factor (Toxo-GIF), which inhibits the intracellular multiplication of Toxoplasma gondii within nonimmune mouse macrophages. Concanavalin A and phytohemagglutinin-P were found to induce vigorous production of Toxo-GIF, whereas bacterial lipopolysaccharide did not. In vitro treatment of splenic lymphocytes with rabbit anti-mouse thymocyte serum plus complement abolished almost completely their ability to produce Toxo-GIF. Treatment of splenic lymphocytes with inhibitors of protein synthesis, cycloheximide or puromycin resulted in a remarkable reduction of the ability of sensitized lymphocytes to produce this lymphokine. Thus the production of Toxo-GIF seems to be dependent on the cellular metabolic events of sensitized T-lymphocytes. The significant activity of Toxo-GIF was demonstrable even in the supernate of lymphocyte cultures incubated in serum-free medium and was also evident after immune lymphocytes and homologous antigen were incubated for the relatively short period of 10 h.
进行了体外评估，以研究产生一种新的淋巴因子（作者称之为弓形虫生长抑制因子，即Toxo - GIF）的免疫淋巴细胞的一些生物学特性，该因子可抑制刚地弓形虫在非免疫小鼠巨噬细胞内的细胞内增殖。发现刀豆球蛋白A和植物血凝素 - P可诱导Toxo - GIF的大量产生，而细菌脂多糖则不能。用兔抗小鼠胸腺细胞血清加补体对脾淋巴细胞进行体外处理，几乎完全消除了它们产生Toxo - GIF的能力。用蛋白质合成抑制剂环己酰亚胺或嘌呤霉素处理脾淋巴细胞，导致致敏淋巴细胞产生这种淋巴因子的能力显著降低。因此，Toxo - GIF的产生似乎依赖于致敏T淋巴细胞的细胞代谢事件。即使在无血清培养基中培养的淋巴细胞培养上清液中，Toxo - GIF的显著活性也可得到证明，并且在免疫淋巴细胞和同源抗原孵育相对较短的10小时后也很明显。
