Mirhaghgoye Jalali S F, Jabbari A R, Esmael Zad M
Pasteurella National Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran.
Department of Central Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran.
Arch Razi Inst. 2017 Sep;72(3):165-171. doi: 10.22092/ari.2017.111607. Epub 2016 Oct 23.
Pasteurella multocida isa gram-negative bacterial pathogen that is causative agent of a wide range of diseases in many animal species and humans. Lipopolysaccharides (LPS) are an important virulence factor, minor changes to structure of which can exert dramatic effects on pathogenicity of P. multocida in its host. LPS can be used for the identification and classification of strains with somatic typing systems.The aim of this study was to identify the LPS genotypes of the ovine P. multocida isolates obtained from pneumonia cases in Iran. The LPS genotype of the isolates was determined using eight specific primers for LPS outer core biosynthesis loci. The LPS genes were amplified by polymerase chain reaction (PCR), then they were sequenced and compared to the sequences registered in the GenBank. Of the 32 ovine P. multocida isolates tested, 21 (65.62%) isolates belonged to genotype L6, 9 (28.12%) isolates contained genotype L3, 1 (3.12%) isolate had both L3 and L6 loci, and 1 (3.12%) isolate remained untypeable. The LPS-PCR was able to type 31 of 32 field ovine isolates from Iran. According to the phylogenetic analysis, L3 genotype isolates were grouped into two distinct lineages. LPS gene sequences among L6 genotypes of ovine P. multocida isolates from Iran and the related sequences in the GenBank were highly similar (>99.5%). LPS-PCR is an accurate genotyping method that was able to classify P. multocida strains into one of the eight distinct LPS genotypes.
多杀性巴氏杆菌是一种革兰氏阴性细菌病原体，是许多动物物种和人类多种疾病的病原体。脂多糖（LPS）是一种重要的毒力因子，其结构的微小变化可对多杀性巴氏杆菌在宿主体内的致病性产生显著影响。LPS可用于通过菌体分型系统对菌株进行鉴定和分类。本研究的目的是鉴定从伊朗肺炎病例中分离出的绵羊多杀性巴氏杆菌菌株的LPS基因型。使用针对LPS外核心生物合成位点的八种特异性引物确定分离株的LPS基因型。通过聚合酶链反应（PCR）扩增LPS基因，然后对其进行测序，并与GenBank中登记的序列进行比较。在测试的32株绵羊多杀性巴氏杆菌分离株中，21株（65.62%）属于L6基因型，9株（28.12%）含有L3基因型，1株（3.12%）同时具有L3和L6位点，1株（3.12%）分离株无法分型。LPS-PCR能够对来自伊朗的32株野外绵羊分离株中的31株进行分型。根据系统发育分析，L3基因型分离株分为两个不同的谱系。来自伊朗的绵羊多杀性巴氏杆菌分离株L6基因型之间的LPS基因序列与GenBank中的相关序列高度相似（>99.5%）。LPS-PCR是一种准确的基因分型方法，能够将多杀性巴氏杆菌菌株分类为八种不同的LPS基因型之一。
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