Rímac Rocío, Luna Luis, Hurtado Raquel, Rosadio Raúl, Maturrano Lenin
Biology and Molecular Genetic Section, Department of Public and Animal Health. Faculty of Veterinary Medicine, San Marcos National University, Lima, Peru.
Department of Animal Husbandry and Agricultural Production, Faculty of Veterinary Medicine, San Marcos National University, Lima, Peru.
Trop Anim Health Prod. 2017 Aug;49(6):1325-1328. doi: 10.1007/s11250-017-1309-5. Epub 2017 May 18.
Pasteurella multocida is a common constituent of upper respiratory tract microbiota but is frequently isolated of alpaca lung tissues from pulmonary infections. Despite its importance, very little is known about this bacteria at molecular level. In order to characterize P. multocida isolates, 24 isolates recovered from 46 mortal acute cases in young alpacas with suspected pneumonia were analyzed, using biochemical and molecular tests for capsule and LPS typing, virulence factors detection, and ERIC-PCR genetic diversity analysis. All the P. multocida isolates belonged to the capsular type A, LPS genotype L6 (related to serotypes 10, 11, 12, and 15), and possessed virulence factors gene toxA and tbpA. ERIC-PCR analysis revealed two electrophoretic profiles, and the majority of isolates (23/24) shared the same fingerprint, indicating strong evidence that there was a common source of infection for all the affect animals. This study revealed the detection of P. multocida type A, LPS genotype L6, and toxA+ and tbpA+ from dead young alpacas with pneumonia in Peru.
多杀性巴氏杆菌是上呼吸道微生物群的常见组成部分，但在患有肺部感染的羊驼肺组织中也经常分离到。尽管其很重要，但在分子水平上对这种细菌的了解却非常少。为了鉴定多杀性巴氏杆菌分离株，对从46例疑似肺炎的年轻羊驼急性死亡病例中分离出的24株菌株进行了分析，采用生化和分子试验进行荚膜和脂多糖分型、毒力因子检测以及ERIC-PCR基因多样性分析。所有多杀性巴氏杆菌分离株均属于A荚膜型、LPS基因型L6（与血清型10、11、12和15相关），并具有毒力因子基因toxA和tbpA。ERIC-PCR分析显示出两种电泳图谱，大多数分离株（23/24）具有相同的指纹图谱，这有力地表明所有受感染动物存在共同的感染源。本研究揭示了在秘鲁死于肺炎的年轻羊驼中检测到A型多杀性巴氏杆菌、LPS基因型L6以及toxA+和tbpA+。
