Department of Food Science and Biotechnology of Animal Resources, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, South Korea.
Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120, South Korea.
Microb Pathog. 2018 Dec;125:486-492. doi: 10.1016/j.micpath.2018.10.018. Epub 2018 Oct 17.
This study aimed to isolate and demonstrate their antioxidant and immunostimulatory activities of potential probiotics. The isolated strains, S. Pum19, SC28, and SC61 showed potential probiotic properties including stability in artificial gastric and bile conditions, non-production of β-glucuronidase, suitable antibiotic susceptibility, and attachment to intestinal cells. S. Pum19, SC28, and SC61 strains were identified as Leuconostoc citreum, Pediococcus pentosaceus, and Lactobacillus paraplantarum, respectively. Of the 3 potential probiotic LAB strains, intact cells of L. paraplantarum SC61 showed higher antioxidant activity, including DPPH radical scavenging, β-carotene bleaching inhibition, reducing power, superoxide anion scavenging, and ABTS radical scavenging activity. In addition, L. paraplantarum SC61 produced the most nitric oxide production and its mRNA expression level for iNOS, IL-1β, IL-6, and TNF-α were superior to those of L. rhamnosus GG. Therefore, L. paraplantarum SC61 was demonstrated to exhibit antioxidant and immunostimulatory activity and to have potential use as a probiotic product.
本研究旨在分离并证明潜在益生菌的抗氧化和免疫刺激活性。分离出的菌株 S. Pum19、SC28 和 SC61 表现出潜在的益生菌特性,包括在人工胃液和胆汁条件下的稳定性、不产生β-葡萄糖醛酸酶、适当的抗生素敏感性以及对肠细胞的黏附性。S. Pum19、SC28 和 SC61 菌株分别被鉴定为柠檬明串珠菌、戊糖片球菌和植物乳杆菌。在 3 株潜在益生菌 LAB 菌株中,完整细胞的植物乳杆菌 SC61 表现出更高的抗氧化活性,包括 DPPH 自由基清除、β-胡萝卜素漂白抑制、还原力、超氧阴离子清除和 ABTS 自由基清除活性。此外,植物乳杆菌 SC61 产生的一氧化氮产量及其 iNOS、IL-1β、IL-6 和 TNF-α 的 mRNA 表达水平均优于鼠李糖乳杆菌 GG。因此,植物乳杆菌 SC61 表现出抗氧化和免疫刺激活性,并具有作为益生菌产品的潜力。