Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Korea.
Int J Mol Sci. 2020 Feb 12;21(4):1227. doi: 10.3390/ijms21041227.
The purpose of this study was to evaluate the probiotic characteristics and neuroprotective effects of bacteria isolated from Korean fermented foods. Three bacterial strains ( KU200060, KU200171, and KU200793) showed potential probiotic properties, such as high tolerance against artificial gastric juice and bile salts, sensitivity to antibiotics, nonproduction of carcinogenic enzymes, and high adhesion to intestinal cells. Heat-killed KU200060 and KU200793 showed higher antioxidant activity than heat-killed KU200171. The conditioned medium (CM) was used to evaluate the reaction between HT-29 cells and each heat-killed strain. All CMs protected SH-SY5Y cells from 1-methyl-4-phenylpyridinium (MPP)-induced toxicity. The expression of brain-derived neurotropic factor (BDNF) mRNA in HT-29 cells treated with CM containing heat-killed KU200793 was the highest. The CM significantly reduced the Bax/Bcl ratio and increased BDNF mRNA expression in SH-SY5Y cells treated with MPP. These results indicate that KU200793 can be used as a prophylactic functional food, having probiotic potential and neuroprotective effects.
本研究旨在评估从韩国发酵食品中分离出的细菌的益生菌特性和神经保护作用。三株细菌（KU200060、KU200171 和 KU200793）具有较高的耐胃酸和胆汁盐能力、对抗生素敏感、不产生致癌酶、对肠道细胞具有高黏附性等潜在益生菌特性。热灭活的 KU200060 和 KU200793 的抗氧化活性高于热灭活的 KU200171。用条件培养基（CM）评估了 HT-29 细胞与各热灭活菌之间的反应。所有 CM 均能保护 HT-29 细胞免受 1-甲基-4-苯基吡啶（MPP）诱导的毒性。含有热灭活 KU200793 的 CM 处理的 HT-29 细胞中脑源性神经营养因子（BDNF）mRNA 的表达最高。CM 显著降低了 MPP 处理的 SH-SY5Y 细胞中 Bax/Bcl 比值，并增加了 BDNF mRNA 的表达。这些结果表明 KU200793 可用作具有益生菌潜力和神经保护作用的预防性功能性食品。
