Kinoshita Y, Fukase M, Nakada M, Fujita T
Biochem Biophys Res Commun. 1987 Apr 29;144(2):763-9. doi: 10.1016/s0006-291x(87)80030-x.
Effects of parathyroid hormone (PTH), low phosphate environment, and 12-O-tetradecanoyl phorbol-13-acetate (TPA) on the phosphate reabsorption by the renal tubular cells from mutant hemizygous hypophosphatemic (Hyp/Y) mice and their littermates (+/Y) were studied using a phosphate accumulation system which had been developed recently. This system mimics phosphate transport at the renal tubules. When cultured in a normal phosphate medium, the characteristics of the phosphate accumulation by Hyp cells was almost identical with that by normal cells; a PTH-induced inhibition and a TPA-induced stimulation of phosphate accumulation. However, when preincubated in a low phosphate medium, the accumulation of phosphate by normal cells increased significantly, while that by Hyp cells did not. These results indicate that the adaptation to the low phosphate environment is defective in Hyp cells and it may be one of the cause of renal phosphate leakage in the Hyp mouse.
利用最近开发的磷酸盐积累系统，研究了甲状旁腺激素（PTH）、低磷环境和12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）对突变半合子低磷血症（Hyp/Y）小鼠及其同窝正常小鼠（+/Y）肾小管细胞磷酸盐重吸收的影响。该系统模拟肾小管的磷酸盐转运。当在正常磷酸盐培养基中培养时，Hyp细胞积累磷酸盐的特性与正常细胞几乎相同；PTH诱导的磷酸盐积累抑制和TPA诱导的磷酸盐积累刺激。然而，当在低磷培养基中预孵育时，正常细胞的磷酸盐积累显著增加，而Hyp细胞则没有。这些结果表明，Hyp细胞对低磷环境的适应性存在缺陷，这可能是Hyp小鼠肾磷酸盐泄漏的原因之一。
