大鼠和兔肾钠氢交换体之间的种属差异。

Species differences between rat and rabbit renal Na+/H+ exchangers.

作者信息

Friedrich T, Sablotni J, Burckhardt G

出版信息

Biochem Biophys Res Commun. 1987 Apr 29;144(2):869-75. doi: 10.1016/s0006-291x(87)80045-1.

Abstract

Amiloride, 5-ethylisopropylamiloride (EIPA), and 5-ethylisopropyl-6-bromo-amiloride (Br-EIPA) inhibit rabbit and rat renal Na+/H+ exchangers with comparable potency. Irreversible inhibitions by Br-EIPA after irradiation of rat and rabbit renal brush-border vesicles are also similar. In contrast, irreversible inhibition by N,N'-dicyclohexylcarbodiimide (DCCD) of the rabbit renal antiporter requires higher DCCD concentrations as compared to the rat. Rabbit renal brush-border membranes show highest [14C]DCCD incorporation at MW 80,000, 51,000 and 36,000 and lack the amiloride-protectable MW 65,000 protein previously identified as Na+/H+ exchanger in rat kidney cortex. The data indicate species differences with respect to renal Na+/H+ exchangers.

摘要

氨氯吡咪、5-乙基异丙基氨氯吡咪（EIPA）和5-乙基异丙基-6-溴氨氯吡咪（Br-EIPA）对兔和大鼠肾脏的Na⁺/H⁺交换体具有相似的抑制效力。大鼠和兔肾刷状缘囊泡经照射后Br-EIPA的不可逆抑制作用也相似。相比之下，与大鼠相比，N,N'-二环己基碳二亚胺（DCCD）对兔肾反向转运体的不可逆抑制需要更高的DCCD浓度。兔肾刷状缘膜在分子量80,000、51,000和36,000处显示出最高的[¹⁴C]DCCD掺入量，并且缺乏先前在大鼠肾皮质中鉴定为Na⁺/H⁺交换体的氨氯吡咪可保护的分子量65,000的蛋白质。数据表明在肾脏Na⁺/H⁺交换体方面存在物种差异。