Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México.
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México.
Arch Med Res. 2018 Jul;49(5):350-355. doi: 10.1016/j.arcmed.2018.10.001. Epub 2018 Oct 17.
We have reported the presence of varicella-zoster virus (VZV) DNA and viral particles in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients during exacerbation. It is not known whether these viruses are infective.
To determine whether the VZV found in CSF of MS patients in exacerbation phase are infective.
VZV found in CSF of MS patients was quantified by qPCR. Vero E6 cell cultures were incubated with CSF of five MS cases positive for VZV DNA, containing herpes-like viral particles. Propagated virus harvested from these cultures were used to infect new VeroE6 cells. Localization of an immediate-early and a late structural VZV proteins was monitored by confocal microscopy after 72 h. CSF from five non-inflammatory neurological (NIN) patients were used as controls.
A cytopathic effect was found in cultured cells inoculated with CSF from MS patients. Both, structural VZV glycoprotein (gB) and immediate-early VZV protein (IE62) were detected in Vero E6 cultures inoculated with samples from all five MS cases. CSF from control patients produced no effect on Vero E6 cells.
When present in the CSF at relapses of MS, VZV is infective under in vitro conditions.
我们曾报道多发性硬化症（MS）患者在疾病恶化期的脑脊液（CSF）中存在水痘-带状疱疹病毒（VZV）DNA 和病毒颗粒。目前尚不清楚这些病毒是否具有感染性。
确定在 MS 患者恶化期 CSF 中发现的 VZV 是否具有感染性。
通过 qPCR 定量 CSF 中的 VZV。用含有疱疹样病毒颗粒的 CSF 孵育来自五例 VZV DNA 阳性 MS 病例的 Vero E6 细胞培养物。从这些培养物中收获的增殖病毒用于感染新的 VeroE6 细胞。在感染后 72 小时，通过共聚焦显微镜监测早期和晚期 VZV 结构蛋白的定位。将来自五例非炎症性神经病变（NIN）患者的 CSF 用作对照。
接种 MS 患者 CSF 的培养细胞出现细胞病变效应。在接种来自所有五例 MS 病例的样本的 Vero E6 培养物中均检测到 VZV 结构糖蛋白（gB）和早期 VZV 蛋白（IE62）。对照患者的 CSF 对 Vero E6 细胞无影响。
在 MS 复发时存在于 CSF 中时，VZV 在体外条件下具有感染性。
