Gurevich A I, Babiĭ N I
Bioorg Khim. 1987 Feb;13(2):213-7.
Effect of deletions downstream from the replication origin on copy number of ColE1 related (pBR322 derived) plasmids has been studied. Along with main control elements (RNAI of defined secondary structure, the repressor protein, effectiveness of promoter upstream of gene of RNA primer) replication is influenced by transcription level propagated into the replicon region from promoters even in distal parts of plasmid. Structure of the region adjacent to the ori is important for startpoint recognition specificity, so that deletion of this region reduces the effectiveness of replication.
已对复制起点下游缺失对ColE1相关（源自pBR322）质粒拷贝数的影响进行了研究。除了主要控制元件（具有特定二级结构的RNAI、阻遏蛋白、RNA引物基因上游启动子的有效性）外，即使在质粒远端部分的启动子转录进入复制子区域的转录水平也会影响复制。与ori相邻区域的结构对于起点识别特异性很重要，因此该区域的缺失会降低复制效率。
