Kumaresan A, Johannisson Anders, Humblot Patrice, Bergqvist Ann-Sofi
Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7054, SE-750 07 Uppsala, Sweden.
Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7054, SE-750 07 Uppsala, Sweden.
Theriogenology. 2019 Jan 15;124:48-56. doi: 10.1016/j.theriogenology.2018.09.028. Epub 2018 Sep 29.
This study was conducted to investigate the complex interactions between oviducts and cryopreserved spermatozoa. Herein we report the dynamic changes in bull sperm functions during in vitro incubation with bovine estrus and luteal oviductal fluid. Frozen-thawed bull spermatozoa was incubated either in non-capacitating medium, capacitating medium, non-capacitating medium containing 20% v/v estrus oviductal fluid or non-capacitating medium containing 20% v/v luteal oviductal fluid for 6 h at 38 °C under 5% CO. At hourly interval spermatozoa were evaluated for kinematics, tyrosine phosphorylation and acrosome reaction. The sperm velocity parameters were higher (P < 0.05) in capacitating medium compared to the other treatments. At 4 and 5 h of incubation, the proportion of live tyrosine phosphorylated spermatozoa was higher (P < 0.05) in estrus oviductal fluid compared to all other treatments. From 4 to 6 h of incubation the proportion of live acrosome reacted spermatozoa was higher (P < 0.05) in estrus oviductal fluid compared to the other treatments. We conclude that estrus oviductal fluid induced tyrosine phosphorylation and acrosome reaction in a higher proportion of frozen-thawed bull spermatozoa compared to luteal oviductal fluid, although sperm kinematics were not significantly influenced by oviductal during incubation.
本研究旨在探讨输卵管与冷冻保存精子之间的复杂相互作用。在此，我们报告了公牛精子在与牛发情期和黄体期输卵管液进行体外孵育期间功能的动态变化。将冻融后的公牛精子在非获能培养基、获能培养基、含20%（v/v）发情期输卵管液的非获能培养基或含20%（v/v）黄体期输卵管液的非获能培养基中于38°C、5%二氧化碳条件下孵育6小时。每隔一小时评估精子的运动学、酪氨酸磷酸化和顶体反应。与其他处理相比，获能培养基中的精子速度参数更高（P<0.05）。在孵育4小时和5小时时，发情期输卵管液中活的酪氨酸磷酸化精子比例高于所有其他处理（P<0.05）。在孵育4至6小时期间，发情期输卵管液中活的顶体反应精子比例高于其他处理（P<0.05）。我们得出结论，与黄体期输卵管液相比，发情期输卵管液能诱导更高比例的冻融公牛精子发生酪氨酸磷酸化和顶体反应，尽管孵育期间输卵管液对精子运动学没有显著影响。
