Knop R H, Carney D N, Chen C W, Cohen J S, Minna J D
Cancer Res. 1987 Jul 1;47(13):3357-9.
Human lung cancers are divided into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) based on established criteria. SCLC differs from NSCLC by the expression of biomarkers, including creatine kinase-BB isoenzyme (EC 2.7.3.2). Subtypes of SCLC are referred to as classic and variant, both of which have elevated levels of creatine kinase-BB isoenzyme. We, therefore, applied 31P nuclear magnetic resonance spectroscopy to cell lines of classic SCLC, variant SCLC, and NSCLC human tumors, using continuous perfusion to identify any differences in the detectable levels of intracellular high-energy phosphate compounds. The spectra indicate that only the variant SCLC cells maintain high levels of phosphocreatine. Additionally, the classic SCLC cells express elevated levels of a diphosphodiester. Neither phosphocreatine nor diphosphodiesters are found in the NSCLC cell spectra.
根据既定标准，人类肺癌分为小细胞肺癌（SCLC）和非小细胞肺癌（NSCLC）。SCLC与NSCLC的区别在于生物标志物的表达，包括肌酸激酶-BB同工酶（EC 2.7.3.2）。SCLC的亚型分为经典型和变异型，两者的肌酸激酶-BB同工酶水平均升高。因此，我们对经典SCLC、变异SCLC和NSCLC人类肿瘤细胞系应用31P核磁共振波谱法，采用连续灌注来确定细胞内高能磷酸化合物可检测水平的任何差异。光谱表明，只有变异SCLC细胞维持高水平的磷酸肌酸。此外，经典SCLC细胞表达的二磷酸二酯水平升高。在NSCLC细胞光谱中未发现磷酸肌酸和二磷酸二酯。
