Guo Hongyue, Wahab M Farooq, Berthod Alain, Armstrong Daniel W
Department of Chemistry and Biochemistry, University of Texas at Arlington, Planetarium Place, Arlington, TX 76019, USA.
Institut des Sciences Analytiques, CNRS, Université de Lyon 1, 5 rue de la Doua, 69100 Villeurbanne, France.
J Pharm Anal. 2018 Oct;8(5):324-332. doi: 10.1016/j.jpha.2018.08.001. Epub 2018 Aug 9.
Current trends in chiral analysis of pharmaceutical drugs are focused on faster separations and higher separation efficiencies. Core-shell or superficially porous particles (SPP) based chiral stationary phases (CSPs) provide reduced analysis times while maintaining high column efficiencies and sensitivity. In this study, mobile phase conditions suitable for chiral analyses with electrospray ionization LC-MS were systematically investigated using vancomycin as a representative CSP. The performance of a 2.7 µm SPP based vancomycin CSP (SPP-V) 10 cm × 0.21 cm column was compared to that of a corresponding 5 µm fully porous particles based analogue column. The results demonstrated that the SPP-V column provides higher efficiencies, 2-5 time greater sensitivity and shorter analysis time for a set of 22 basic pharmaceutical drugs. The SPP-V was successfully applied for the analysis of the degradation products of racemic citalopram whose enantiomers could be selectively identified by MS.
当前药物手性分析的趋势集中在更快的分离速度和更高的分离效率上。基于核壳或表面多孔颗粒（SPP）的手性固定相（CSP）在保持高柱效和灵敏度的同时，可缩短分析时间。在本研究中，以万古霉素作为代表性的CSP，系统地研究了适用于电喷雾电离LC-MS手性分析的流动相条件。将一根10 cm×0.21 cm的基于2.7 µm SPP的万古霉素CSP（SPP-V）柱的性能与一根相应的基于5 µm全多孔颗粒的类似柱进行了比较。结果表明，对于一组22种碱性药物，SPP-V柱具有更高的柱效、高2-5倍的灵敏度和更短的分析时间。SPP-V已成功应用于外消旋西酞普兰降解产物的分析，其对映体可通过质谱选择性鉴定。
