Cronin M J, Weiss J, Rogol A D, Baertschi A J
Endocr Res. 1987;13(1):85-95. doi: 10.1080/07435808709023665.
We examined the ability of four commonly used culture media to support prolactin (PRL), growth hormone (GH), and adrenocorticotropic hormone (ACTH) release, as well as the inhibitory PRL response to dopamine. After a week of primary culture, rat anterior pituitary cells from both genders were studied over a 4 hour period. Whereas ACTH secretion was similar across the various media, PRL and GH release were lessened with M199 and F10, respectively. Dopamine inhibited PRL release under all media conditions which was inconsistant with the reported lack of a dopamine effect with RPMI-1640 medium. These data confirm the postulate that media culture conditions can determine the degree of expression of constituitive phenotypes in anterior pituitary cells.
我们检测了四种常用培养基支持催乳素（PRL）、生长激素（GH）和促肾上腺皮质激素（ACTH）释放的能力，以及PRL对多巴胺的抑制反应。原代培养一周后，对来自两性的大鼠垂体前叶细胞进行了4小时的研究。虽然不同培养基中ACTH的分泌相似，但在M199和F10培养基中，PRL和GH的释放分别减少。在所有培养基条件下，多巴胺均抑制PRL释放，这与报道的RPMI-1640培养基中缺乏多巴胺效应不一致。这些数据证实了以下假设：培养基培养条件可决定垂体前叶细胞组成型表型的表达程度。
