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在立方形RAC乳腺细胞系中转染int-1乳腺癌基因会导致形态转化和致瘤性。

Transfection of the int-1 mammary oncogene in cuboidal RAC mammary cell line results in morphological transformation and tumorigenicity.

作者信息

Rijsewijk F, van Deemter L, Wagenaar E, Sonnenberg A, Nusse R

出版信息

EMBO J. 1987 Jan;6(1):127-31. doi: 10.1002/j.1460-2075.1987.tb04729.x.

DOI:10.1002/j.1460-2075.1987.tb04729.x
PMID:3034569
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553367/
Abstract

The int-1 gene is often activated by proviral insertion in mouse mammary tumors. Direct evidence for the normal function of this gene and its role in tumorigenesis has therefore been lacking. To examine possible biological effects of int-1 activation in in vitro cell systems, we have constructed recombinant molecules of genomic int-1 DNA, transcriptionally activated by retroviral promoters. Transfection of these constructs into cuboidal RAC311C mammary cells leads to morphological transformation of the cells and rapid tumorigenicity.

摘要

int-1基因在小鼠乳腺肿瘤中常因前病毒插入而被激活。因此，一直缺乏该基因正常功能及其在肿瘤发生中作用的直接证据。为了研究int-1激活在体外细胞系统中可能产生的生物学效应，我们构建了由逆转录病毒启动子转录激活的基因组int-1 DNA重组分子。将这些构建体转染到立方形的RAC311C乳腺细胞中会导致细胞形态转化和快速致瘤性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c21/553367/6a337494fe37/emboj00241-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c21/553367/f18b6243d9f0/emboj00241-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c21/553367/682e435ffbc3/emboj00241-0128-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c21/553367/49fad09915af/emboj00241-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c21/553367/6a337494fe37/emboj00241-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c21/553367/f18b6243d9f0/emboj00241-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c21/553367/682e435ffbc3/emboj00241-0128-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c21/553367/49fad09915af/emboj00241-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c21/553367/6a337494fe37/emboj00241-0130-a.jpg

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