Kutlay O
Bratisl Lek Listy. 2018;119(10):625-629. doi: 10.4149/BLL_2018_111.
Apelin is an endogenous adipocytokine that plays an important role in the regulation of cardiovascular function. Apelin-36 is a member of apelin family. However cardiac reports related to apelin-36 are very rare. The purpose of this study is to investigate the impact of apelin-36 on hemodynamic variables of the isolated rat hearts.
Twenty-eight rats were equally divided into four groups: control, apelin-36 at the following concentrations: 1 nM, 10 nM and 100 nM. The isolated hearts were perfused with modified Krebs-Henseleit solution (mK-Hs) by using the Langendorff method. Cardiac parameters, including left ventricular developed pressure (LVDP), maximal rate of pressure development (+dP/dtmax), heart rate (HR) and coronary flow (CF) were measured. Gene expressions relevant to cardiomyocyte contractility were determined by real-time PCR.
10 and 100 nM doses of apelin-36 perfusion led to positive inotropy with an increase of LVDP and +dP/dtmax, which are the indexes of cardiac contractility. Furthermore both doses of apelin-36 increased endothelial nitric oxide synthase (eNOS), sarco/endoplasmic reticulum Ca2+-ATPase (Serca2a) and β2-Adrenergic receptors (β2-AR) mRNA.
These results showed that apelin-36 had a positive inotropic effect on the isolated rat heart and can induce eNOS, Serca2a and β2-AR genes activation that enhances contractility of the heart (Tab. 1, Fig. 2, Ref. 23).
Apelin是一种内源性脂肪细胞因子，在心血管功能调节中起重要作用。Apelin-36是Apelin家族的成员。然而，与Apelin-36相关的心脏研究报告非常罕见。本研究的目的是探讨Apelin-36对离体大鼠心脏血流动力学变量的影响。
28只大鼠平均分为四组：对照组、1 nM、10 nM和100 nM浓度的Apelin-36组。采用Langendorff法用改良的Krebs-Henseleit溶液（mK-Hs）灌注离体心脏。测量心脏参数，包括左心室舒张末压（LVDP）、最大压力上升速率（+dP/dtmax）、心率（HR）和冠状动脉血流量（CF）。通过实时PCR测定与心肌细胞收缩性相关的基因表达。
10 nM和100 nM剂量的Apelin-36灌注导致正性肌力作用，LVDP和+dP/dtmax增加，这是心脏收缩性的指标。此外，两种剂量的Apelin-36均增加了内皮型一氧化氮合酶（eNOS）、肌浆网/内质网Ca2+-ATP酶（Serca2a）和β2-肾上腺素能受体（β2-AR）的mRNA。
这些结果表明，Apelin-36对离体大鼠心脏具有正性肌力作用，并可诱导eNOS、Serca2a和β2-AR基因激活，增强心脏收缩力（表1，图2，参考文献23）。
