Onrat Serap Tutgun, Onrat Ersel, Ercan Onay Emine, Yalım Zafer, Avşar Alaettin
1 Department of Medical Genetics, Faculty of Medicine, Afyon Kocatepe University , Afyonkarahisar, Turkey .
2 Department of Cardiology, Faculty of Medicine, Afyon Kocatepe University , Afyonkarahisar, Turkey .
Genet Test Mol Biomarkers. 2018 Nov;22(11):644-651. doi: 10.1089/gtmb.2018.0188. Epub 2018 Oct 26.
Most dilated cardiomyopathies are either an ischemic dilated cardiomyopathy (IsDC) or an idiopathic dilated cardiomyopathy (IdDC). The treatments for both IsDC and IdDC are of a similar nature (upwards of 90%). Coronary revascularization, however, is only feasible for IsDC. The purpose of this study was to determine if microRNAs (miRNAs) could be used as biomarkers to distinguish between IsDC and IdDC.
Patients were divided into two groups: IsDC and IdDC, with 25 patients in each group, and 10 healthy persons serving as a control group. In our study, the following miRNA expressions were detected using the Rotor Gene Q real-time polymerase chain reaction cycler (Qiagen) for all IsDC and IdDC subjects: let-7b-5p, let-7c-5p, miR-1-3p, miR-15b-5p, miR-17-5p, miR-19a-3p, miR-19b-3p, miR-20a-5p, miR-20b-5p, miR-23a-3p, miR-24-3p, miR-27a-3p, miR-28-5p, miR-30e-5p, miR-99b-5p, miR-100-5p, miR-101-3p, miR-103a-3p, miR-106a-5p, miR-125b-5p, miR-126-3p, miR-126-5p, miR-140-5p, miR-191-5p, miR-195-5p, miR-199a-3p, miR-214-3p, miR-222-3p, miR-342-3p, and miR-378a-3p.
We found that miR-24-3p, miR-28-5p, miR-100-5p, miR-103-3p, miR-125b5p, miR-214-3p, let-7b-5p, and let-7c-5p were each overexpressed by more than twofold in both the IsDC and IdDC groups when compared to the controls. We also found that miR-15b-5p and miR-106a-5p may be used to distinguish between patients with IsDC and IdDC.
Our study has demonstrated that miR-15b-5p and miR-106a-5p expression levels could potentially serve as useful biomarkers for distinguishing between IsDC and IdDC.
大多数扩张型心肌病要么是缺血性扩张型心肌病（IsDC），要么是特发性扩张型心肌病（IdDC）。IsDC和IdDC的治疗方法性质相似（超过90%）。然而，冠状动脉血运重建仅对IsDC可行。本研究的目的是确定微小RNA（miRNA）是否可作为区分IsDC和IdDC的生物标志物。
患者分为两组：IsDC组和IdDC组，每组25例患者，10名健康人作为对照组。在我们的研究中，使用Rotor Gene Q实时聚合酶链反应循环仪（Qiagen）对所有IsDC和IdDC受试者检测以下miRNA表达：let-7b-5p、let-7c-5p、miR-1-3p、miR-15b-5p、miR-17-5p、miR-19a-3p、miR-19b-3p、miR-20a-5p、miR-20b-5p、miR-23a-3p、miR-24-3p、miR-27a-3p、miR-28-5p、miR-30e-5p、miR-99b-5p、miR-100-5p、miR-101-3p、miR-103a-3p、miR-106a-5p、miR-125b-5p、miR-126-3p、miR-126-5p、miR-140-5p、miR-191-5p、miR-195-5p、miR-199a-3p、miR-214-3p、miR-222-3p、miR-342-3p和miR-378a-3p。
我们发现，与对照组相比，miR-24-3p、miR-28-5p、miR-100-5p、miR-103-3p、miR-125b5p、miR-214-3p、let-7b-5p和let-7c-5p在IsDC组和IdDC组中均有超过两倍的过表达。我们还发现miR-15b-5p和miR-106a-5p可用于区分IsDC和IdDC患者。
我们的研究表明，miR-15b-5p和miR-106a-5p的表达水平可能作为区分IsDC和IdDC的有用生物标志物。
