Liang Q F, Du X H, Su G Y, Zhang Y, Wang Z Q, Cao K
Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing 100005, China.
Zhonghua Yan Ke Za Zhi. 2018 Oct 11;54(10):767-774. doi: 10.3760/cma.j.issn.0412-4081.2018.10.009.
To study the relationship between genetic typing and the antibiotic susceptibility of () isolated from keratitis or conjunctivitis patients. Experimental study. Thirty-four (34) strains of were isolated from 34 cases of keratitis or conjunctivitis. The genomic DNA was extracted and amplified with PCR. With the method of multi locus sequences typing (MLST), gene fragments from 7 house-keeping genes were amplified and the products were sequenced. The results were submitted to the MLST website (www.pubmlst.org). In comparison with the allele of the corresponding gene, the allele spectrums of the strain were obtained with 7 housekeeping genes. At last, the MLST genotypes of the isolated strains were determined. With the START software, the evolutionary tree was established with UPGMA method. With the microdilution method, the MIC(90) of 13 antimicrobial agents was determined. The MIC(90) value of antimicrobial agents among different genotypes of was comparatively analyzed. Ten (10) genotypes were obtained from 34 strains of . The dominant types were ST239, ST2592 and ST188. The clustering of genotyping was relatively concentrated, mainly in group Ⅰ (25 strains of , 83.3% of the total), and followed by group Ⅱ (5 strains of , 16.7%). The conjunctival isolates were distributed in the subgroup A of group Ⅰ. The cornea isolates were concentrated in subgroup B and group Ⅱ. With the exact probability method, the × chi square tests were used as statistic analysis method. The difference between the bacterial genotyping of two sources was statistically significant (0.011). Twenty-four strains of in group Ⅰ was sensitive to Vancomycin, Rifampicin and Amikacin (sensitivity ratio was 24/24, 20/24 and 20/24, respectively), and was generally resistant to other antibiotics. The values of MIC(90) of ciprofloxacin, ofloxacin, gatifloxacin and moxifloxacin to in subgroup A (0.16±0.07, 0.51±0.42, 0.31±0.14, 0.22±0.33) were significantly lower than the values in subgroup B(0.74±0.11, 0.84±0.45, 0.67±0.03, 0.68±0.26). The difference was statistically significant (0.004, 0.026, 0.034, 0.001). There was no significant difference between the MIC(90) values of the other 9 kinds of antibiotics in the subgroup A and in the subgroup B of ( value 0.047-0.561). The genotype of of corneal isolations and conjunctival isolations were different. The conjunctival isolates were distributed in the subgroup A of group Ⅰ and the corneal isolates were concentrated in subgroup B and group Ⅱ. There is a significant correlation between the MLST genotypes and antibiotic sensitivity. -.
研究从角膜炎或结膜炎患者中分离出的（）的基因分型与抗生素敏感性之间的关系。实验研究。从34例角膜炎或结膜炎患者中分离出34株（）。提取基因组DNA并通过PCR进行扩增。采用多位点序列分型（MLST）方法，扩增7个管家基因的基因片段并对产物进行测序。将结果提交至MLST网站（www.pubmlst.org）。与相应基因的等位基因进行比较，获得该菌株7个管家基因的等位基因谱。最后确定分离菌株的MLST基因型。使用START软件，采用UPGMA方法构建进化树。采用微量稀释法测定13种抗菌药物的MIC（90）。比较分析不同基因型（）中抗菌药物的MIC（90）值。从34株（）中获得10种基因型。优势型为ST239、ST2592和ST188。基因分型聚类相对集中，主要在Ⅰ组（25株，占总数的83.3%），其次是Ⅱ组（5株，占16.7%）。结膜分离株分布于Ⅰ组的A亚组。角膜分离株集中在B亚组和Ⅱ组。采用确切概率法，使用×卡方检验作为统计分析方法。两种来源的细菌基因分型差异具有统计学意义（0.011）。Ⅰ组的24株（）对万古霉素、利福平、阿米卡星敏感（敏感率分别为24/24、20/24和20/24），对其他抗生素普遍耐药。环丙沙星、氧氟沙星、加替沙星和莫西沙星对A亚组（）的MIC（90）值（0.16±0.07、0.51±0.42、0.31±0.14、0.22±0.33）显著低于B亚组（0.74±0.11、0.84±0.45、0.67±0.03、0.68±0.26）。差异具有统计学意义（0.004、0.026、0.034、0.001）。（）A亚组和B亚组中其他9种抗生素的MIC（90）值之间无显著差异（值0.047 - 0.561）。角膜分离株和结膜分离株的（）基因型不同。结膜分离株分布于Ⅰ组的A亚组，角膜分离株集中在B亚组和Ⅱ组。MLST基因型与抗生素敏感性之间存在显著相关性。- 。
