长链非编码 RNA PVT1 的下调可减弱胶质细胞瘤细胞对紫杉醇的耐药性。
Downregulation of long noncoding RNA PVT1 attenuates paclitaxel resistance in glioma cells.
机构信息
Department of Clinical Laboratory, The Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang, China.
Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang, China.
出版信息
Cancer Biomark. 2018;23(3):447-453. doi: 10.3233/CBM-181573.
BACKGROUND
Drug resistance in clinical cancer treatment has become an issue.
OBJECTIVE
We focus on abnormally expressed lncRNAs in glioma and investigating the function of PVT1.
METHODS
The paclitaxel-resistant glioma cells SHG-44 RE was obtained through screening the SHG 44 cells that were cultured in medium containing a certain concentration of paclitaxel. Cell survival of SHG 44 RE and SHG 44 cells under the treatment of paclitaxel was detected by MTT assay. The aberrant expressed lncRNAs were screened out with microarray analysis. Further qRT-PCR was utilized to validate the expression of lncRNA PVT1 in the two cells. After manipulating the expression of PVT1, cell viability and apoptosis were measured by MTT and flow cytometry respectively.
RESULTS
LncRNA PVT1 was overexpressed in glioma cells SHG-44 RE compared with parent SHG-44 cells. Down-regulation of lncRNA PVT1 inhibited the SHG-44 RE cell viability and increased glioma SHG-44 RE cells apoptosis after paclitaxel treatment, suggesting that inhibition of lncRNA PVT1 improved paclitaxel sensibility in human glioma cells.
CONCLUSION
Down-regulation of PVT1 could enhance chemosensitivity of paclitaxel, induce apoptosis of glioma cells and noteworthy inhibit glioma cells proliferation. Our findings of PVT1 could contribute to attenuate paclitaxel resistance in clinical medicine.
背景
癌症治疗中的药物耐药性已成为一个问题。
目的
我们专注于胶质瘤中异常表达的 lncRNAs，并研究 PVT1 的功能。
方法
通过在含有一定浓度紫杉醇的培养基中培养 SHG-44 细胞来获得紫杉醇耐药性胶质瘤细胞 SHG-44RE。用 MTT 法检测紫杉醇处理后 SHG-44RE 和 SHG-44 细胞的存活情况。用微阵列分析筛选出异常表达的 lncRNAs。进一步用 qRT-PCR 验证两种细胞中 lncRNA PVT1 的表达。在操纵 PVT1 的表达后，用 MTT 和流式细胞术分别测量细胞活力和细胞凋亡。
结果
与亲本 SHG-44 细胞相比，lncRNA PVT1 在胶质瘤细胞 SHG-44RE 中过表达。下调 lncRNA PVT1 抑制了 SHG-44RE 细胞的活力，并增加了紫杉醇处理后胶质瘤 SHG-44RE 细胞的凋亡，表明抑制 lncRNA PVT1 提高了人胶质瘤细胞对紫杉醇的敏感性。
结论
下调 PVT1 可以增强紫杉醇的化疗敏感性，诱导胶质瘤细胞凋亡，并显著抑制胶质瘤细胞增殖。我们对 PVT1 的研究结果可能有助于减轻临床实践中的紫杉醇耐药性。
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