苜蓿根瘤菌ntrA（rpoN）基因对于多种代谢功能是必需的。
Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions.
作者信息
Ronson C W, Nixon B T, Albright L M, Ausubel F M
出版信息
J Bacteriol. 1987 Jun;169(6):2424-31. doi: 10.1128/jb.169.6.2424-2431.1987.
Abstract
We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.
摘要
我们报道了苜蓿根瘤菌ntrA样（glnF rpoN）基因的鉴定与克隆，并表明苜蓿根瘤菌ntrA产物（NtrA）对于C4-二羧酸转运、硝酸盐同化以及共生固氮作用而言是必需的。DNA序列分析表明，苜蓿根瘤菌NtrA与肺炎克雷伯氏菌NtrA具有38%的同源性。亚克隆及互补分析表明，苜蓿根瘤菌ntrA启动子位于起始密码子的125个碱基对范围内，可能为组成型表达。
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