Dedrick Elsie A, Reyda Florian B, Iwanyckyj Elise K, Ruhnke Timothy R
Biology Department & Biological Field Station, State University of New York, College at Oneonta, Ravine Parkway Oneonta, NY, USA.
Department of Biology, West Virginia State University, Institute, WV, USA.
Folia Parasitol (Praha). 2018 Sep 21;65:2018.014. doi: 10.14411/fp.2018.014.
Morphological and molecular analyses of cestode specimens collected during survey work of batoid elasmobranchs and their parasites in Senegal revealed two new species of the rhinebothriidean cestode genus Stillabothrium Healy et Reyda 2016. Stillabothrium allisonae Dedrick et Reyda sp. n. and Stillabothrium charlotteae Iwanyckyj, Dedrick et Reyda sp. n. are both described from Fontitrygon margaritella (Compagno et Roberts) and Fontitrygon margarita (Günther). Both new cestode species overlap in geographic distribution, host use and proglottid morphology, but are distinguished from each other, and from the other seven described species of Stillabothrium, on the basis of their pattern of bothridial loculi. Phylogenetic analyses based on sequence data for 1,084 bp from the D1-D3 region of 28S rDNA that included multiple specimens of both new species and eight other species of Stillabothrium corroborated the morphologically-determined species boundaries. The phylogenetic analyses indicate that S. allisonae sp. n. and S. charlotteae sp. n. are sister species, a noteworthy pattern given that the two species of the stingray genus Fontitrygon they both parasitise, F. margaritella and F. margarita, are also sister species. Although species of Stillabothrium vary widely in their patterns of facial loculi, the variation does not appear to correlate with phylogeny. Most species of Stillabothrium parasitise myliobatiform elasmobranch genera of the Dasyatidae Jordan. This study brings the number of described species of Stillabothrium to nine, three of which occur in the eastern Atlantic, two of which occur off the northern coast of Australia, and four of which are from coastal Borneo.
在对塞内加尔的鳐形板鳃亚纲及其寄生虫进行调查工作期间收集的绦虫标本的形态学和分子分析揭示了rhinebothriidean绦虫属Stillabothrium Healy et Reyda 2016的两个新物种。Allisonae Stillabothrium Dedrick et Reyda新种和Charlotteae Stillabothrium Iwanyckyj, Dedrick et Reyda新种均从珍珠前口蝠鲼（Compagno et Roberts）和玛格丽塔前口蝠鲼（Günther）中描述而来。这两个新的绦虫物种在地理分布、宿主利用和节片形态上重叠，但根据它们的吸槽小孔模式，它们彼此之间以及与Stillabothrium的其他七个已描述物种区分开来。基于28S rDNA D1-D3区域1084 bp序列数据的系统发育分析，其中包括这两个新物种的多个标本以及Stillabothrium的其他八个物种，证实了形态学确定的物种界限。系统发育分析表明，Allisonae Stillabothrium新种和Charlotteae Stillabothrium新种是姐妹物种，鉴于它们共同寄生的前口蝠鲼属的两个物种，即珍珠前口蝠鲼和玛格丽塔前口蝠鲼，也是姐妹物种，这是一个值得注意的模式。尽管Stillabothrium物种的面部小孔模式差异很大，但这种差异似乎与系统发育无关。Stillabothrium的大多数物种寄生于约旦的鲼科的鲼形板鳃亚纲属。这项研究使Stillabothrium的已描述物种数量增加到九个，其中三个出现在东大西洋，两个出现在澳大利亚北部海岸外，四个来自婆罗洲海岸。
