James Harris D, Halajian Ali, Santos Joana L, Swanepoel Lourens H, Taylor Peter John, Xavier Raquel
CIBIO/InBIO, Centro de Investigacao em Biodiversidade e Recursos Geneticos, Campus Agrario de Vairao, Vairao, Portugal.
Departamento de Biologia, Faculdade de Ciencias da Universidade do Porto, Porto, Portugal.
Folia Parasitol (Praha). 2018 Oct 2;65:2018.015. doi: 10.14411/fp.2018.015.
Tissue samples from wildlife from South Africa were opportunistically collected and screened for haemoprotozoan parasites using nonspecific PCR primers. Samples of 127 individuals were tested, comprising over 50 different species. Haemogregarines were the most commonly identified parasites, but sarcocystids and piroplasmids were also detected. Phylogenetic analyses estimated from the 18S rDNA marker highlighted the occurrence of several novel parasite forms and the detection of parasites in novel hosts. Phylogenetic relationships, which have been recently reviewed, appear to be much more complex than previously considered. Our study highlights the high diversity of parasites circulating in wildlife in this biodiverse region, and the need for further studies to resolve taxonomic issues.
对来自南非野生动物的组织样本进行了机会性采集，并使用非特异性PCR引物对血原虫寄生虫进行筛查。对127个个体的样本进行了检测，涵盖50多个不同物种。血簇虫是最常鉴定出的寄生虫，但也检测到了肉孢子虫和梨形虫。基于18S rDNA标记进行的系统发育分析突出了几种新型寄生虫形式的出现以及在新宿主中检测到寄生虫。最近经过综述的系统发育关系似乎比之前认为的要复杂得多。我们的研究突出了在这个生物多样性地区野生动物中传播的寄生虫的高度多样性，以及进一步开展研究以解决分类学问题的必要性。
