Asher L V, Binn L N, Marchwicki R H
J Virol Methods. 1987 Mar;15(4):323-8. doi: 10.1016/0166-0934(87)90155-8.
Virus-like particles were demonstrated by electron microscopy in BS-C-1 cells infected with hepatitis A virus (HAV). Particles were usually enclosed within vesicles and accompanied by myelin-like membranous structures. Less often they were seen free in the cytoplasm. They were never observed in the nucleus. By immunoperoxidase staining particles were found to contain HAV antigens. These antigens were also found in the membrane of the vesicles surrounding the masses of particles and adjacent parts of the mitochondrial membranes. Our results demonstrate the usefulness of an electron microscopic immunocytochemical technique to study replication of HAV.
用电子显微镜在感染甲型肝炎病毒（HAV）的BS-C-1细胞中证实了病毒样颗粒。颗粒通常包被在囊泡内，并伴有髓鞘样膜性结构。较少见的是它们游离于细胞质中。从未在细胞核中观察到它们。通过免疫过氧化物酶染色发现颗粒含有HAV抗原。这些抗原也存在于围绕颗粒团块的囊泡膜以及线粒体膜的相邻部分中。我们的结果证明了电子显微镜免疫细胞化学技术在研究HAV复制中的有用性。
