Miller Cecelia R, Heerema Nyla A
Department of Pathology, The Ohio State University, Columbus, OH, USA.
Methods Mol Biol. 2019;1881:27-34. doi: 10.1007/978-1-4939-8876-1_3.
Chromosome analysis of chronic lymphocytic leukemia (CLL) is an important clinical tool for evaluating prognosis and disease progression. Visualizing chromosomes microscopically using traditional cytogenetic techniques requires dividing cells to be arrested during metaphase. The major challenge for performing this analysis on CLL samples is stimulating the cells to divide in culture. Stimulation of CLL cells with CpG oligodeoxynucleotides has improved our ability to perform chromosome analysis for this leukemia. This protocol should help the reader successfully culture CLL samples for clinical chromosome analysis.
慢性淋巴细胞白血病（CLL）的染色体分析是评估预后和疾病进展的一项重要临床工具。使用传统细胞遗传学技术在显微镜下观察染色体需要将处于分裂期的细胞阻断。对CLL样本进行这项分析的主要挑战在于刺激细胞在培养中进行分裂。用CpG寡脱氧核苷酸刺激CLL细胞提高了我们对这种白血病进行染色体分析的能力。本方案应有助于读者成功培养CLL样本以进行临床染色体分析。
