通过灵敏的液相色谱-串联质谱法检测脐带组织中的子宫内大麻暴露情况。
Detection of In Utero Cannabis Exposure in Umbilical Cord Tissue by a Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method.
作者信息
Wu Fang, Jensen Triniti L, McMillin Gwendolyn A
机构信息
Department of Pathology, University of Utah, Salt Lake City, UT, USA.
ARUP Laboratories, Salt Lake City, UT, USA.
出版信息
Methods Mol Biol. 2019;1872:211-222. doi: 10.1007/978-1-4939-8823-5_20.
In utero exposure to cannabis may cause various short- and long-term health problems in newborns, such as low birth weight and neonatal withdrawal syndrome. Drug testing with umbilical cord tissue can be used to identify in utero exposure to cannabis. Here, we described a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method that simultaneously quantifies four cannabinoids in umbilical cord tissue, including tetrahydrocannabinol (THC), 11-nor-Δ9-carboxy-THC (THC-COOH), cannabinol (CBN), and 11-hydroxy-THC (11-OH-THC). Umbilical cord specimens are weighed and homogenized, and cannabinoids are extracted using anion exchange solid-phase extraction columns (AX-SPE). Liquid chromatography separation is performed, and quantitative results are obtained using LC-MS/MS.
子宫内接触大麻可能会导致新生儿出现各种短期和长期健康问题，如低出生体重和新生儿戒断综合征。通过脐带组织进行药物检测可用于识别子宫内大麻接触情况。在此，我们描述了一种液相色谱-串联质谱（LC-MS/MS）方法，该方法可同时定量脐带组织中的四种大麻素，包括四氢大麻酚（THC）、11-去甲-Δ9-四氢大麻酚羧酸（THC-COOH）、大麻酚（CBN）和11-羟基-THC（11-OH-THC）。对脐带标本进行称重并匀浆，然后使用阴离子交换固相萃取柱（AX-SPE）提取大麻素。进行液相色谱分离，并使用LC-MS/MS获得定量结果。
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