Shaheen Shabnum, Jaffer Mehwish, Aslam Barira, Hussain Khadim, Khan Asaf, Ashfaq Muhammad, Ulfat Mobina, Sardar Andaleeb Anwar, Ahmad Mushtaq, Zafar Muhammad, Khan Farah
Lahore College for Women University, Lahore, Pakistan.
Bioinformatics and Biotechnology, Government College university, Faisalabad, Pakistan.
Microsc Res Tech. 2018 Dec;81(12):1501-1505. doi: 10.1002/jemt.23150. Epub 2018 Oct 23.
Current study was focused to examine the total bacterial count in packed and unpacked ice cream and kulfa collected from 12 different localities of Lahore. The bacterial colonies were isolated and grown on agar-broth media under sterilized conditions. Serial dilution technique was used to compose the replicates to get total viable count of bacteria. Results in case of packed ice cream samples indicated maximum (618 × 10 CFU/g) and minimum (79 × 10 CFU/g) bacterial count while in case of unpacked ice cream samples maximum and minimum bacterial count was 163 × 10 CFU/g and 71 × 10 CFU/g, respectively. Whereas in case of packed kulfa samples, maximum and minimum recorded bacterial count was 163 × 10 CFU/g and 72 × 10 CFU/g, respectively. The LM and SEM of the isolated bacteria were also performed for correct identification. Results indicated that the total bacterial count recorded in the samples exceeded the standard tolerable range which can lead to serious health damage of consumers.
当前的研究聚焦于检测从拉合尔12个不同地区采集的包装和未包装的冰淇淋及库尔法中的细菌总数。细菌菌落被分离出来,并在无菌条件下在琼脂肉汤培养基上生长。采用系列稀释技术制备重复样本以获得细菌的总活菌数。包装冰淇淋样本的结果显示,细菌计数最高为(618×10 CFU/g),最低为(79×10 CFU/g);而未包装冰淇淋样本的最高和最低细菌计数分别为163×10 CFU/g和71×10 CFU/g。对于包装的库尔法样本,记录的最高和最低细菌计数分别为163×10 CFU/g和72×10 CFU/g。还对分离出的细菌进行了光学显微镜和扫描电子显微镜观察以进行正确鉴定。结果表明,样本中记录的细菌总数超过了标准可容忍范围,这可能会对消费者的健康造成严重损害。