3-甲基-4-硝基苯酚对小鼠卵巢和睾丸细胞增殖、凋亡和卵母细胞成熟的毒性作用。

Toxicological effects of 3-methyl-4-nitrophenol on mouse ovarian and testicular cell proliferation, apoptosis and oocyte maturation.

机构信息

College of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, People's Republic of China.

School of Biological Sciences, University of Hong Kong, Hong Kong, China.

出版信息

Reprod Toxicol. 2018 Dec;82:94-102. doi: 10.1016/j.reprotox.2018.10.005. Epub 2018 Oct 21.

DOI:10.1016/j.reprotox.2018.10.005

PMID:30352283

Abstract

The aim of the present study was to reveal the effects of 3-methyl-4-nitrophenol (PNMC) on mouse gonadal cell proliferation and apoptosis. Immature female and male mice were intramuscularly injected with 100 mg/kg PNMC or vehicle every five days. One-month later, ovarian rather than testicular weights were significantly decreased. The positive terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling staining was enhanced in both testicular seminiferous epithelium and ovarian corpus luteum. Further study in ovaries showed that PNMC treatment increased the protein expressions of PCNA, p21 and p27, and decreased the expression of Cyclin D2. Whereas PNMC had no significantly influence on those protein expressions in testes. In addition, we demonstrated that 100 nM PNMC significantly suppressed mouse oocyte meiotic resumption and cumulus cell expansion in vitro. These findings suggest that the reproductive toxicities of PNMC involve affecting the processes of gonadal cell apoptosis and proliferation.

摘要

本研究旨在揭示 3-甲基-4-硝基苯酚（PNMC）对小鼠性腺细胞增殖和凋亡的影响。将未成年雌性和雄性小鼠肌肉内注射 100mg/kg PNMC 或载体，每五天一次。一个月后，卵巢重量而非睾丸重量显著降低。阳性末端脱氧核苷酸转移酶介导的 dUTP-地高辛缺口末端标记染色在睾丸曲细精管上皮和卵巢黄体中均增强。进一步在卵巢中的研究表明，PNMC 处理增加了 PCNA、p21 和 p27 的蛋白表达，并降低了 Cyclin D2 的表达。然而，PNMC 对睾丸中这些蛋白表达没有显著影响。此外，我们证明 100nM PNMC 显著抑制了体外小鼠卵母细胞减数分裂恢复和卵丘细胞扩张。这些发现表明，PNMC 的生殖毒性涉及影响性腺细胞凋亡和增殖的过程。