Michelassi F, Shahinian H K, Ferguson M K
J Surg Res. 1987 May;42(5):475-82. doi: 10.1016/0022-4804(87)90021-7.
Arachidonic acid is metabolized to leukotriene (LT) B4, C4, D4, and E4 by lipoxygenase. LTB4 is a chemotactic agent while LTC4 and LTD4 stimulate smooth muscle fibers to contract. Mesenteric vessels have the capacity to release leukotrienes. The possibility that leukotrienes might be responsible for or contribute to mesenteric ischemia during mesenteric low flow, embolism, and thrombosis prompted us to investigate their action on mesenteric vessels. LTB4, C4, and D4 were applied topically on small bowel mesentery of 22 Sprague-Dawley rats in sequentially increasing concentrations. Mesenteric arterioles with diameters of 8-20 microns were observed through a microscope and vessel diameters were measured using a video shear monitor. LTB4 had no effect on diameter, but doses as low as 3 X 10(-8) M induced white blood cell adherence to venular endothelium, reflecting the potent chemotactic properties of this compound. LTC4 and D4 had no effect on systemic blood pressure or white blood cell adherence. Applications of 6.4 X 10(-9), 3.2 X 10(-8), and 6.4 X 10(-8) M LTC4 decreased mesenteric arteriolar diameter to 85.3* +/- 4.7% (mean +/- SD), 75.7* +/- 7.5%, and 66.8* +/- 6.1% of baseline, and 4 X 10(-9), 2 X 10(-8), and 4 X 10(-8) M LTD4 decreased diameter to 84.9* +/- 6.1%, 75.1* +/- 4.2%, and 64.1* +/- 5% of baseline, respectively (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
花生四烯酸通过脂氧合酶代谢为白三烯（LT）B4、C4、D4和E4。LTB4是一种趋化剂，而LTC4和LTD4刺激平滑肌纤维收缩。肠系膜血管有释放白三烯的能力。白三烯可能在肠系膜低流量、栓塞和血栓形成期间导致或促成肠系膜缺血，这促使我们研究它们对肠系膜血管的作用。将LTB4、C4和D4以浓度递增的方式局部应用于22只Sprague-Dawley大鼠的小肠系膜。通过显微镜观察直径为8 - 20微米的肠系膜小动脉，并使用视频剪应力监测仪测量血管直径。LTB4对直径无影响，但低至3×10⁻⁸ M的剂量可诱导白细胞黏附于小静脉内皮，反映出该化合物强大的趋化特性。LTC4和D4对全身血压或白细胞黏附无影响。应用6.4×10⁻⁹、3.2×10⁻⁸和6.4×10⁻⁸ M的LTC4可使肠系膜小动脉直径分别降至基线的85.3%±4.7%、75.7%±7.5%和66.8%±6.1%，而4×10⁻⁹、2×10⁻⁸和4×10⁻⁸ M的LTD4可使直径分别降至基线的84.9%±6.1%、75.1%±4.2%和64.1%±5%（P<0.05）。（摘要截断于250字）
