Nizyaeva N V, Kulikova G V, Nagovitsyna M N, Kan N E, Prozorovskaya K N, Shchegolev A I
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2018 Oct;165(6):793-797. doi: 10.1007/s10517-018-4267-7. Epub 2018 Oct 23.
The expression of microRNA-17, microRNA-181a, and microRNA-519a in the villous tree in preeclampsia was analyzed using chromogenic in situ hybridization technique (CISH). It was found that in early-onset preeclampsia, the expression of microRNA-17 in the syncytiotrophoblast was higher (p<0.05) than in late preeclampsia, and the expression of microRNA-519a was higher (p<0.05) than in women with preterm birth at 26-31 weeks gestation. We revealed higher level of expression of microRNA-181a (p<0.05) in the cytoplasm of the syncytiotrophoblast of intermediate placental villi in the group with premature delivery in comparison with early preeclampsia. In full-term pregnancy, the expression of microRNA-181a in the vascular endothelium of placental villi was higher (p<0.02) than in women with premature deliveries. Analysis of the target genes associated with these microRNAs showed that damage to the trophoblast typical of preeclampsia, especially up to 34 weeks gestation, was accompanied by selective activation of genes participating in invasion and compensatory suppression of oncoprotective genes associated with the development of malignant neoplasms.
采用显色原位杂交技术（CISH）分析子痫前期绒毛树中微小RNA - 17、微小RNA - 181a和微小RNA - 519a的表达。结果发现，早发型子痫前期合体滋养层中微小RNA - 17的表达高于晚发型子痫前期（p<0.05），微小RNA - 519a的表达高于孕26 - 31周早产的女性（p<0.05）。与早发型子痫前期相比，早产组中间胎盘绒毛合体滋养层细胞质中微小RNA - 181a的表达水平更高（p<0.05）。足月妊娠时，胎盘绒毛血管内皮中微小RNA - 181a的表达高于早产女性（p<0.02）。对与这些微小RNA相关的靶基因分析表明，子痫前期典型的滋养层损伤，尤其是妊娠34周前，伴随着参与侵袭的基因的选择性激活以及与恶性肿瘤发生相关的肿瘤保护基因的代偿性抑制。
