The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China.
BGI-Shenzhen, Shenzhen, 518083, China.
Amino Acids. 2019 Feb;51(2):345-353. doi: 10.1007/s00726-018-2670-z. Epub 2018 Oct 23.
The diversity of defensive peptides from skin of amphibians has been demonstrated. These peptides may have resulted from the diversity of microorganisms encountered by amphibians. In this study, peptidomics and RNA sequencing analyses were used to study deeply the defensive peptides of the skin secretions from Polypedates megacephalus. A total of 99 defensive peptides have been identified from the skin secretions. Among these peptides, 3 peptides were myotropical peptides and 34 peptides classified as protease inhibitor peptides. 5 lectins, 8 antimicrobial peptides, 26 immunomodulatory peptides, 10 wound-healing peptides and 13 other bioactive peptides were identified as belonging to the innate immune system. One antimicrobial peptide Pm-amp1 showed high similarity to antimicrobial peptide marcin-18. This peptide was successfully expressed and showed moderate activity against four tested strains. These identified peptides highlight the extensive diversity of defensive peptides and provide powerful tools to understand the defense weapon of frog.
已经证明了来自两栖动物皮肤的防御肽的多样性。这些肽可能是由两栖动物遇到的微生物多样性产生的。在这项研究中，使用肽组学和 RNA 测序分析深入研究了大蹼铃蟾皮肤分泌物中的防御肽。从皮肤分泌物中鉴定出了 99 种防御肽。其中，3 种肽是肌肉肽，34 种肽归类为蛋白酶抑制剂肽。鉴定出 5 种凝集素、8 种抗菌肽、26 种免疫调节肽、10 种伤口愈合肽和 13 种其他生物活性肽，它们都属于先天免疫系统。一种抗菌肽 Pm-amp1 显示出与抗菌肽 marcin-18 的高度相似性。该肽成功表达并显示对四种测试菌株具有中等活性。这些鉴定出的肽突出了防御肽的广泛多样性，并为了解青蛙的防御武器提供了有力的工具。
