Rowe Joshua A, Morandi Federica, Osborne Dustin R, Wall Jonathan S, Kennel Stephen J, Reed Robert B, LeBlanc Amy K
College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee.
Graduate School of Medicine, University of Tennessee, Knoxville, Tennessee.
Anat Histol Embryol. 2019 Jan;48(1):46-52. doi: 10.1111/ahe.12410. Epub 2018 Oct 23.
3'-deoxy-3'-[ F]fluorothymidine ( FLT) is a radiopharmaceutical tracer used with positron emission tomography (PET), often in combination with computed tomography (CT), to image DNA synthesis, and thus, cellular proliferation. Characteristic accumulation of the tracer within haematopoietic bone marrow provides a noninvasive means to assess marrow activity and distribution throughout the living animal. The present study utilizes three-dimensional analysis of FLT-PET/CT scans to quantify the relative skeletal distribution of active marrow by anatomic site in the dog. Scans were performed on six healthy, adult (3-6 years of age), mixed-breed dogs using a commercially available PET/CT scanner consisting of a 64-slice helical CT scanner combined with an integrated four ring, high-resolution LSO PET scanner. Regions of interest encompassing 11 separate skeletal regions (skull, cervical vertebral column, thoracic vertebral column, lumbar vertebral column, sacrum, ribs, sternum, scapulae, proximal humeri, ossa coxarum, and proximal femora) were manually drawn based on CT images and thresholded by standardized uptake value to delineate bone marrow activity. Activity within each skeletal region was then divided by the total skeletal activity to derive the per cent of overall marrow activity within an individual site. The majority of proliferative marrow was located within the vertebral column. Of the sites traditionally accessed clinically for marrow sampling, the proximal humerus contained the largest percentage, followed by the ossa coxarum, proximal femur, and sternum, respectively. This information may be used to guide selection of traditional marrow sampling sites as well as inform efforts to spare important sites of haematopoiesis in radiation therapy planning.
3'-脱氧-3'-[F]氟胸苷（FLT）是一种放射性药物示踪剂，用于正电子发射断层扫描（PET），通常与计算机断层扫描（CT）联合使用，以成像DNA合成，从而反映细胞增殖情况。示踪剂在造血骨髓中的特征性积聚提供了一种非侵入性手段，可用于评估活体动物体内骨髓的活性和分布。本研究利用FLT-PET/CT扫描的三维分析，按解剖部位对犬体内活跃骨髓的相对骨骼分布进行量化。使用一台商用PET/CT扫描仪对6只健康的成年（3至6岁）杂种犬进行扫描，该扫描仪由一台64层螺旋CT扫描仪与一台集成的四环高分辨率LSO PET扫描仪组成。根据CT图像手动绘制包含11个独立骨骼区域（颅骨、颈椎、胸椎、腰椎、骶骨、肋骨、胸骨、肩胛骨、近端肱骨、髋骨和近端股骨）的感兴趣区域，并通过标准化摄取值进行阈值处理，以描绘骨髓活性。然后将每个骨骼区域内的活性除以总骨骼活性，得出单个部位在总体骨髓活性中所占的百分比。大部分增殖性骨髓位于脊柱内。在临床上传统的骨髓采样部位中，近端肱骨所含比例最大，其次分别是髋骨、近端股骨和胸骨。这些信息可用于指导传统骨髓采样部位的选择，以及在放射治疗计划中为保留重要造血部位提供参考。
