在F9小鼠胚胎癌细胞中对病毒增强子功能的阻遏物和激活物进行的竞争研究。
Competition studies with repressors and activators of viral enhancer function in F9 mouse embryonal carcinoma cells.
作者信息
Sleigh M J, Lockett T J, Kelly J, Lewy D
出版信息
Nucleic Acids Res. 1987 May 26;15(10):4307-24. doi: 10.1093/nar/15.10.4307.
Abstract
DNA competition studies have been used to investigate the presence of a repressor of viral enhancer function in F9 mouse embryonal carcinoma cells. The complete polyoma virus enhancer region, cotransfected into F9 cells with the SV40 promoter/enhancer attached to a chloramphenicol acetyl transferase marker gene, induced a small increase in pSV2CAT expression. This can be explained by preferential but weak binding by polyoma sequences of a molecule repressing pSV2CAT transcription. Repressor activity substantially disappeared when the cells were induced to differentiate by retinoic acid. Repressor binding was localised to one half of the polyoma enhancer, but was lost on further fragmentation of this region. It appears that multiple sequence elements may be required for repressor binding and that these are at least partially separable from the complement of elements binding enhancer activating molecules.
摘要
DNA竞争研究已被用于调查F9小鼠胚胎癌细胞中病毒增强子功能阻遏物的存在情况。完整的多瘤病毒增强子区域，与连接氯霉素乙酰转移酶标记基因的SV40启动子/增强子共转染到F9细胞中，诱导pSV2CAT表达略有增加。这可以通过多瘤序列对抑制pSV2CAT转录的分子的优先但弱结合来解释。当细胞用视黄酸诱导分化时，阻遏物活性基本消失。阻遏物结合定位于多瘤增强子的一半，但在该区域进一步片段化时丧失。似乎阻遏物结合可能需要多个序列元件，并且这些元件至少部分地与结合增强子激活分子的元件互补物分开。
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本文引用的文献
1
Does the major histocompatibility complex serve as a specific receptor for Semliki Forest virus?主要组织相容性复合体是否作为Semliki森林病毒的特异性受体？
J Virol. 1980 Apr;34(1):256-65. doi: 10.1128/JVI.34.1.256-265.1980.
2
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
3
Characterization of enhancer elements in the long terminal repeat of Moloney murine sarcoma virus.莫洛尼鼠肉瘤病毒长末端重复序列中增强子元件的特性分析。
J Virol. 1984 Jan;49(1):183-9. doi: 10.1128/JVI.49.1.183-189.1984.
4
Common control of the heat shock gene and early adenovirus genes: evidence for a cellular E1A-like activity.热休克基因与早期腺病毒基因的共同调控：细胞中类似E1A活性的证据。
Mol Cell Biol. 1984 May;4(5):867-74. doi: 10.1128/mcb.4.5.867-874.1984.
5
A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.一种淋巴细胞特异性细胞增强子位于免疫球蛋白重链基因连接区的下游。
Cell. 1983 Jul;33(3):729-40. doi: 10.1016/0092-8674(83)90015-6.
6
Non-function of a Moloney murine leukaemia virus regulatory sequence in F9 embryonal carcinoma cells.莫洛尼鼠白血病病毒调控序列在F9胚胎癌细胞中的无功能状态
Nature. 1984;308(5958):470-2. doi: 10.1038/308470a0.
7
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8
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9
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10
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