Graham M Y, Otani T, Boime I, Olson M V, Carle G F, Chaplin D D
Nucleic Acids Res. 1987 Jun 11;15(11):4437-48. doi: 10.1093/nar/15.11.4437.
A cosmid clone containing the entire hCG beta gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis) method. Analysis of this cosmid clone shows that there are 6 hCG beta genes in human genomic DNA. A previously uncloned portion of the hCG beta cluster, termed the "gap" region, has been shown not to contain any sequences homologous to the hCG beta cDNA. The restriction mapping method employed in this study takes advantage of the superior resolution of FIGE for high molecular weight DNA fragments in the size range 15-50 kb. This method is broadly applicable and permits rapid and accurate restriction mapping for extended regions of genomic DNA that have been cloned into cosmid or lambda vectors.
一个包含完整人绒毛膜促性腺激素β基因簇的黏粒克隆已被分离出来。该克隆的限制性图谱已通过间接末端标记场反转凝胶电泳（FIGE）方法确定。对这个黏粒克隆的分析表明，人类基因组DNA中有6个人绒毛膜促性腺激素β基因。人绒毛膜促性腺激素β基因簇中一个以前未克隆的部分，称为“缺口”区域，已被证明不包含与人绒毛膜促性腺激素β互补DNA同源的任何序列。本研究中使用的限制性图谱绘制方法利用了FIGE对大小在15 - 50 kb范围内的高分子量DNA片段的卓越分辨率。该方法具有广泛的适用性，可对已克隆到黏粒或λ载体中的基因组DNA扩展区域进行快速准确的限制性图谱绘制。
