Yu Lan, Guo Rongkai, Jiang Yeqin, Ye Xinghuo, Yang Zhihong, Meng Yijun, Shao Chaogang
a College of Life Sciences , Huzhou University , Huzhou P.R. China.
b Shanghai Institute of Plant Physiology and Ecology , Chinese Academy of Sciences , Shanghai China.
Biosci Biotechnol Biochem. 2019 Feb;83(2):233-242. doi: 10.1080/09168451.2018.1536513. Epub 2018 Oct 25.
MicroRNAs (miRNAs) are important and ubiquitous regulators of gene expression in eukaryotes. However, the information about miRNAs population and their regulatory functions involving in soybean seed development remains incomplete. Base on the Dicer-like1-mediated cleavage signals during miRNA processing could be employed for novel miRNA discovery, a genome-wide search for miRNA candidates involved in seed development was carried out. As a result, 17 novel miRNAs, 14 isoforms of miRNA (isomiRs) and 31 previously validated miRNAs were discovered. These novel miRNAs and isomiRs represented tissue-specific expression and the isomiRs showed significantly higher abundance than that of their miRNA counterparts in different tissues. After target prediction and degradome sequencing data-based validation, 13 novel miRNA-target pairs were further identified. Besides, five targets of 22-nt iso-gma-miR393h were found to be triggered to produce secondary trans-acting siRNA (ta-siRNAs). Summarily, our results could expand the repertoire of miRNAs with potentially important functions in soybean.
微小RNA（miRNA）是真核生物中重要且普遍存在的基因表达调控因子。然而，关于大豆种子发育过程中miRNA群体及其调控功能的信息仍不完整。基于Dicer样1介导的miRNA加工过程中的切割信号可用于发现新的miRNA，我们进行了全基因组搜索以寻找参与种子发育的miRNA候选物。结果，发现了17个新的miRNA、14个miRNA异构体（isomiRs）和31个先前已验证的miRNA。这些新的miRNA和isomiRs表现出组织特异性表达，并且isomiRs在不同组织中的丰度明显高于其对应的miRNA。经过靶标预测和基于降解组测序数据的验证，进一步鉴定出13对新的miRNA-靶标对。此外，发现22-nt的iso-gma-miR393h的五个靶标被触发产生次级反式作用小干扰RNA（ta-siRNAs）。总之，我们的结果可以扩展在大豆中具有潜在重要功能的miRNA库。
