Dvorianchikov G A, Pospelova T V, Tomilin N V
Tsitologiia. 1987 Mar;29(3):331-7.
Mouse fibroblasts NIH 3T3 were transfected with the plasmid pBPV (142-6) containing full genome of bovine papilloma virus 1, and focuses of morphological transformation were selected 2-3 weeks later. DNA molecules, containing BPV-1 sequences, were isolated from extrachromosomal fraction of transformed clones suggesting stable autonomous replication of BPV in 3T3 NIH cells. In some rescued plasmids deletions spanning E6, 7 genes of BPV were found. It is suggested that these genes are not essential for morphological transformation and autonomous replication in 3T3 NIH cells. BPV-transformed clones are able to grow in the medium containing low concentration (0.5%) of serum.
将含有牛乳头瘤病毒1全基因组的质粒pBPV(142 - 6)转染小鼠成纤维细胞NIH 3T3，2 - 3周后筛选出形态转化灶。从转化克隆的染色体外组分中分离出含有BPV - 1序列的DNA分子，表明BPV在NIH 3T3细胞中稳定自主复制。在一些拯救的质粒中发现了跨越BPV E6、E7基因的缺失。提示这些基因对于NIH 3T3细胞的形态转化和自主复制并非必需。BPV转化的克隆能够在含有低浓度(0.5%)血清的培养基中生长。
