Shin Yeseul, Paek Jayoung, Son Ah Young, Kim Hongik, Kook Joong-Ki, Paek Woon Kee, Chang Young Hyo
1ABS Research Support Center/Korean Collection for Type Cultures, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
2Vitabio, Inc., Daejeon, 305-500, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Dec;68(12):3869-3873. doi: 10.1099/ijsem.0.003074. Epub 2018 Oct 25.
Strain YH-tan31 is a Gram-stain-positive, obligately anaerobic, spore-forming and mesophilic bacterium. The cells are rod-shaped and motile by means of peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Clostridium and is most closely related to Clostridium oryzae DSM 28571 (97 % sequence identity), followed by Clostridium cellulovorans DSM 3052 (92.4 %) and Clostridium butyricum KCTC 5387 (92.1 %). The G+C content of the isolate was 30.9 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant fatty acids were C14 : 0, C16 : 0, C16 : 0 DMA,and C19 : 0cis 11,12 DMA. The major end products of glucose fermentation were butyrate, acetate and lactate. This isolate represents a new species within the genus Clostridium, for which we propose the name Clostridiumcomposti sp. nov. (type strain, YH-tan31; =KCTC 15630=JCM 32793).
菌株YH-tan31是革兰氏阳性、专性厌氧、产芽孢的嗜温细菌。细胞呈杆状，借助周生鞭毛运动。基于16S rRNA基因序列的系统发育分析表明，该分离株属于梭菌属，与米梭菌DSM 28571关系最为密切（序列同一性为97%），其次是食纤维梭菌DSM 3052（92.4%）和丁酸梭菌KCTC 5387（92.1%）。该分离株的G+C含量为30.9 mol%。主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。主要脂肪酸为C14 : 0、C16 : 0、C16 : 0 DMA和C19 : 0顺式11,12 DMA。葡萄糖发酵的主要终产物为丁酸、乙酸和乳酸。该分离株代表梭菌属内的一个新物种，我们将其命名为堆肥梭菌（Clostridiumcomposti sp. nov.）（模式菌株YH-tan31；=KCTC 15630=JCM 32793）。
