Effros R M, Dodek P, Edwards J
Ann Biomed Eng. 1987;15(2):189-99. doi: 10.1007/BF02364054.
A new procedure for quantitating endothelial receptor sites is described. The mean transit time of the passage of an appropriate radio-labeled ligand from the artery to vein of an organ is compared to the mean transit times of a vascular indicator and a water label. The concentration of the unlabeled ligand is progressively increased to define that concentration at which half of the enzyme sites are occupied. Values are calculated for the number of receptor sites accessible during a single circulation in each ml of exchangeable tissue water. This approach is illustrated by estimating the sites of carbonic anhydrase present on the pulmonary endothelium, utilizing labeled acetazolamide as a ligand. Preliminary studies of the receptor sites on isolated endothelial cells suspended in an elutriator are also presented.
本文描述了一种定量内皮细胞受体位点的新方法。将合适的放射性标记配体从器官动脉到静脉的平均通过时间与血管指示剂和水标记物的平均通过时间进行比较。逐步增加未标记配体的浓度，以确定占据一半酶位点时的浓度。计算每毫升可交换组织水中单次循环期间可及受体位点的数量。以标记的乙酰唑胺作为配体，通过估计肺内皮细胞上碳酸酐酶的位点来说明这种方法。还介绍了对悬浮在淘析器中的分离内皮细胞上受体位点的初步研究。
