Department of Cell Biology, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.
Department of Physics, Department of Applied Physics, Yale University, 217 Prospect Street, New Haven, CT 06511, USA.
Methods. 2019 Mar 15;157:15-27. doi: 10.1016/j.ymeth.2018.10.009. Epub 2018 Oct 22.
Nuclear morphology is indicative of cellular health in many contexts. In order to robustly and quantitatively measure nuclear size and shape, numerous experimental methods leveraging fluorescence microscopy have been developed. While these methods are useful for quantifying two-dimensional morphology, they often fail to accurately represent the three-dimensional structure of the nucleus, thus omitting important spatial and volumetric information. To address the need for a more accurate image analysis modality, we have developed a software platform that faithfully reconstructs membrane surfaces in three dimensions with sub-pixel resolution. Here, we showcase its broad applicability across species and nuclear scale, as well as provide information on how to employ this platform for diverse experimental systems.
核形态在许多情况下都能反映细胞的健康状况。为了稳健且定量地测量核的大小和形状，已经开发出了许多利用荧光显微镜的实验方法。虽然这些方法对于量化二维形态很有用，但它们往往无法准确表示核的三维结构，从而遗漏了重要的空间和体积信息。为了解决更准确的图像分析模式的需求，我们开发了一个软件平台，该平台能够以亚像素分辨率忠实重建三维的膜表面。在这里，我们展示了它在不同物种和核尺度上的广泛适用性，并提供了有关如何将该平台应用于不同实验系统的信息。
